Influence of Carbon Source, Bile Salts and Incubation Temperature on Recovery of Enterobacteriaceae from Foods Using MacConkey-type Agars

Journal of Food Protection
D A MosselFrancine VAN Rossem

Abstract

The plea made many years ago (27) to replace the ill-defined coli-aerogenes ('coliform') bacteria as indicator organisms in foods processed for safety with the Enterobacteriaceae which are taxonomically accurately defined and as a rule more abundant has of late been more generally accepted. This called for development of a rigorously standardized formula for violet red bile glucose agar and for assessment of the optimal incubation temperature. Four reference strains of Enterobacteriaceae . 120 samples of minced meat and 100 samples of frozen broiler chickens were used in these studies. Considerable differences in the performance of commercially available dried formulae, when used as poured plates were observed. These applied both to productivity and to the type of colony produced by a given pure culture. As expected, replacement of lactose plus glucose by an equimolar amount of glucose did not influence the performance of the medium. Intrinsic toxicity of some batches of medium to non-stressed Enterobacteriaceae appeared to be mainly responsible for substandard performance. It could be overcome by careful selection of the preparations of crystal violet and particularly the bile salts (39) used in the formulae. Incubation at 30 ...Continue Reading

Citations

Oct 1, 1980·The Journal of Applied Bacteriology·S K TammingaE H Kampelmacher
Dec 1, 1980·The Journal of Applied Bacteriology·D A MosselI Eelderink
Dec 1, 1993·The Veterinary Quarterly·H A UrlingsL G van Gils
Dec 1, 1993·The Veterinary Quarterly·M J WeijtensM H Biesheuvel
Mar 28, 2002·Applied and Environmental Microbiology·A LeclercqP Baylac
Aug 6, 2016·Journal of the Science of Food and Agriculture·Naimeh KhazaeiZahra Emam-Djomeh
Oct 26, 2005·Journal of Food Protection·Nikolaos PournisIoannis N Savvaidis
Apr 1, 1981·Applied and Environmental Microbiology·M E Stiles, L K Ng
Apr 1, 1985·Applied and Environmental Microbiology·J P Petzel, P A Hartman
Nov 1, 1980·Applied and Environmental Microbiology·L L Lembke, R N Kniseley

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