Abstract
The immunoglobulin M antibody response to the lipoteichoic acid (LTA) of Staphylococcus aureus ATCC 6538P was examined by a procedure in which erythrocytes sensitized with periodate-activated LTA were used for the detection of immunoglobulin M-producing plaque-forming cells LTA-specific plaque-forming cells were first detected 2 days after immunization with heat-killed bacterial cells, and maximal numbers of plaque-forming cells, mostly of the immunoglobulin M class rather than the immunoblogulin G or immunoglobulin A class, were attained by day 4; specificity for LTA was affirmed by plaque inhibition tests. No plaque-forming cells were found in mice given isolated LTA over a 10,000-fold range of immunizing doses. Mice pretreated with a carrier known to activate thymus-derived helper lymphocytes produced a plaque-forming cell response to LTA only when immunized with LTA bound to the same carrier. This suggests that carrier-specific thymus-derived cells are needed to initiate an antibody response to poorly immunogenic LTA. Since an antibody response can be elicited in mice given heat-killed cells, other cell wall and/or cell membrane constituents may play an important role as immunologically active carriers for this antigen.
References
May 1, 1979·The Journal of Experimental Medicine·V J PasanenP J Baker
Mar 28, 1975·Science·A J Wicken, K W Knox
Jun 1, 1976·Infection and Immunity·B A Fiedel, R W Jackson
Mar 1, 1975·Carbohydrate Research·J ColeyJ Baddiley
Apr 1, 1974·Cellular Immunology·H Braley-Mullen, G C Sharp
Nov 1, 1968·Journal of Bacteriology·K Yoshida, R D Ekstedt
Jun 1, 1973·Journal of Ultrastructure Research·D Van DrielK W Knox
Feb 1, 1971·Immunochemistry·C J Sanderson, D V Wilson
Apr 1, 1972·Cellular Immunology·P Byfield
Oct 1, 1974·Journal of Bacteriology·E HuffT S Theodore
Jun 1, 1973·Bacteriological Reviews·K W Knox, A J Wicken
Apr 1, 1972·The Biochemical Journal·P ToonJ Baddiley
Jul 1, 1972·Infection and Immunity·K W Knox, A J Wicken
Jan 1, 1973·Immunochemistry·F W Chorpenning, H B Stamper
Sep 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·M M Burger
Jun 1, 1966·Journal of Bacteriology·M Moskowitz
Mar 1, 1970·Journal of General Microbiology·M J HewettA J Wicken
Dec 1, 1968·The Biochemical Journal·A R ArchibaldD Button
Jan 1, 1980·Journal of Immunological Methods·P R BeiningP J Baker
Mar 1, 1971·Infection and Immunity·T Matsuno, H D Slade
Feb 28, 1938·The Journal of Experimental Medicine·R J Dubos