PMID: 7527997Oct 1, 1994Paper

Influence of cationic antibiotics on phase behavior of rough-form lipopolysaccharide

Applied Biochemistry and Biotechnology
S Fukuoka, I Karube

Abstract

The rough-form lipopolysaccharide (LPS) interacted with cationic antibiotic polymyxin B and gramicidin S in solution, and showed altered thermotropic phase behavior and viscoelasticity. The phase behavior was measured by differential scanning calorimetry and quartz crystal microbalance (QCM). Addition of polymyxin B of up to 0.5 mg/mL to the 5.0 mg/mL LPS solution increased gel-to-liquid crystalline phase transition enthalpy (delta H) and raised the transition temperature (tmax). The further addition of polymyxin B reduced the delta H value. Gramicidin S produced a different effect, whereby a minor addition reduced tmax and delta H value of the LPS. The LPS film on the platinum electrode of the QCM indicated a downward shift of resonant frequency and an upward shift of resonant resistance when in contact with the antibiotic solution. An interpretation of these variations is that the LPS on the QCM electrode changed not only film weight, but also viscoelasticity owing to contact with the antibiotic solution. The different effects between the antibiotics between polymyxin B and gramicidin S on the LPS are induced by the difference of the governing effect. Polymyxin B interacts with the LPS electrostatically, whereas gramicidin S ...Continue Reading

References

Oct 1, 1976·Immunochemistry·D C Morrison, D M Jacobs
Jan 1, 1990·Annual Review of Biochemistry·C R Raetz
Dec 1, 1990·Applied Biochemistry and Biotechnology·S FukuokaI Karube
May 9, 1990·Biochimica Et Biophysica Acta·M VaaraH Nikaido
May 10, 1974·Annals of the New York Academy of Sciences·M E Bayer
Apr 12, 1983·Biochemistry·R T CoughlinE J McGroarty

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