Influence of cell shape and surface charge on attachment of Mycoplasma pneumoniae to glass surfaces.

Journal of Bacteriology
J FeldnerI Kahane

Abstract

Attachment of Mycoplasma pneumoniae to glass is reduced in the presence of protein, and fatty acid-free bovine serum albumin is more effective than Cohn fraction V. Cultures in the early log phase (pH 7.45 to 7.25) and cultures in the stationary or decline phase (pH 6.9 to 6.4) were more sensitive to this inhibiting effect of protein-containing buffer. Treatment of the glass surface with bovine serum albumin, concanavalin A, or polylysine reduced attachment of the mycoplasma cells. The inhibiting effects of both proteins in buffer or on the glass surface could be overcome by the addition of glucose. Modification of the mycoplasma surface charge by blocking of carboxyl groups or neutralization of ionic lipids by tetracaine altered the attachment level, whereas fibronectin and its corresponding antiserum were without effect. The results suggest that the mycoplasma interaction with glass is a complex multifactorial process. In protein-free buffer both hydrophobic and electrostatic forces are involved; in protein-containing fluid, other factors seem to be involved. The energy required for this type of attachment could be necessary for maintenance of cell shape or synthesis of polypeptides.

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Citations

Jan 1, 1991·Archives of Microbiology·K Gruber, U B Sleytr
Nov 27, 2019·FEMS Microbiology Reviews·Tom E P Kimkes, Matthias Heinemann
Jan 1, 1991·Annals of the New York Academy of Sciences·J A Nadel
Apr 1, 1996·Molecular Microbiology·D C Krause
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Kevin D Young
Dec 9, 2016·Advanced Healthcare Materials·Yevgen KovalenkoCaitlin Howell
Dec 13, 2019·Biochemical Society Transactions·Paul Richard Jesena Yulo, Heather Lyn Hendrickson
Feb 1, 1986·Applied and Environmental Microbiology·R G Cluss, N L Somerson
Mar 30, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Ke Li, Huilian Ma

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