Influence of Cellular Microenvironment on Human Articular Chondrocyte Cell Signaling.

Cartilage
ShihJye TanBo Han

Abstract

Alteration of the cellular microenvironment may influence the intra- and intercellular communication and contribute to cartilage injury and repair. The purpose of this study was to investigate how matrix elasticity/stiffness affects chondrogenic activities, including cell survival, phenotypic expression, and the release of both pro- and anti-inflammatory cytokines. Human articular chondrocytes (HACs) cultured on traditional 2-dimensional (2D) plastic surfaces were compared with those cultured within 3D hydrogel matrices of varying stiffness. Chondrogenic proliferation, differentiation, and the expression of pro- and anti-inflammatory cytokines were evaluated. Both interleukin-1-beta (IL-1β) and human synovial fluid-derived cells (hSFCs) were introduced to study the effects of matrix stiffness on chondrocyte response. Cells demonstrated the most robust chondrogenic differentiation and secreted the least pro-inflammatory cytokines when the matrix stiffness was close to their native microenvironment. The IL-1β effects were attenuated when HACs were co-cultured with hSFCs. Modifying the matrix stiffness to mimic the native cartilage microenvironment not only optimized chondrogenic expression but also was essential for the regulatio...Continue Reading

References

Mar 1, 1995·Canadian Journal of Physiology and Pharmacology·J E Fewell
Jan 19, 2002·The American Journal of Sports Medicine·Lars PetersonAnders Lindahl
Dec 26, 2003·Biophysical Journal·Adam EnglerDennis Discher
Mar 16, 2005·Proceedings of the National Academy of Sciences of the United States of America·Hyun Joon KongDavid J Mooney
Nov 19, 2005·Science·Dennis E DischerYu-Li Wang
Apr 1, 2006·Advanced Drug Delivery Reviews·Dobrila NesicPierre Mainil-Varlet
Aug 23, 2006·Cell·Adam J EnglerDennis E Discher
May 24, 2007·Tissue Engineering·K G Auw YangW J A Dhert
Dec 19, 2007·Methods in Molecular Medicine·Bruno Delorme, Pierre Charbord
May 9, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yasunori Mishima, Martin Lotz
Sep 18, 2009·Tissue Engineering. Part C, Methods·Kenrick KuwaharaBo Han
Nov 12, 2009·Tissue Engineering. Part a·Elena SchuhNicole Rotter
Dec 2, 2010·Nature Reviews. Rheumatology·Mohit KapoorHassan Fahmi
Dec 8, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ichiro SekiyaTakeshi Muneta
Jul 17, 2012·Osteoarthritis and Cartilage·M M J CaronT J M Welting
Mar 22, 2013·Current Rheumatology Reports·Ru Liu-Bryan
Dec 20, 2013·Regenerative Medicine·Josephine FangBo Han
Jun 25, 2014·BioMed Research International·Yue GaoQuanyi Guo
Jan 27, 2015·Progress in Biophysics and Molecular Biology·T C Hodgman, I Ajmera
Feb 18, 2015·Stem Cell Research & Therapy·Eduardo Branco de SousaDiego Pinheiro Aguiar
Feb 14, 2015·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jianjun FangJingbo Yin
Feb 14, 2007·Soft Matter·Ilya LeventalPaul A Janmey

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