Influence of cholesterol on electroporation in lipid membranes of giant vesicles.

European Biophysics Journal : EBJ
Mohammad Abu Sayem KaralShareef Ahammed

Abstract

Irreversible electroporation (IRE) is primarily a nonthermal ablative technology that uses a series of high-voltage and ultra-short pulses with high-frequency electrical energy to induce cell death. This paper presents the influence of cholesterol on the IRE-induced probability of pore formation and the rate constant of pore formation in giant unilamellar vesicles (GUVs). The GUVs are prepared by a mixture of dioleoylphosphatidylglycerol (DOPG), dioleoylphosphatidylcholine (DOPC) and cholesterol using the natural swelling method. An IRE signal of frequency 1.1 kHz is applied to the membranes of GUVs. The probability of pore formation and the rate constant of pore formation events are obtained using statistical analysis from several single GUVs. The time-dependent fraction of intact GUVs among all those examined is fitted to a single exponential decay function from where the rate constant of pore formation is calculated. The probability of pore formation and the rate constant of pore formation decreases with an increase in cholesterol content in the membranes of GUVs. Theoretical equations are fitted to the tension-dependent rate constant of pore formation and to the probability of pore formation, which allows us to obtain the l...Continue Reading

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Citations

Nov 28, 2020·European Biophysics Journal : EBJ·Mohammad Abu Sayem KaralMuhammad Samir Ullah
Apr 29, 2021·The European Physical Journal. E, Soft Matter·Marzuk AhmedMuhammad Samir Ullah
May 15, 2021·PloS One·Malay Kumar SarkarSayed Ul Alam Shibly
Jan 29, 2022·PloS One·Mohammad Abu Sayem KaralShareef Ahammed

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