Influence of chronic inflammation on the malignant phenotypes and the plasticity of colorectal cancer cells.

Biochemistry and Biophysics Reports
Sho WatanabeKiichiro Tsuchiya

Abstract

Sporadic adenoma or adenocarcinoma is often detected during endoscopic surveillance of patients with ulcerative colitis (UC). However, it is occasionally difficult to distinguish these neoplasms from dysplasia or colitis-associated cancers because of the influence of inflammation. However, the influence of inflammation on sporadic neoplasms is not well characterised. To assess this influence, we established a long-term inflammation model of colon cancer cells by inflammatory stimulation with tumour necrosis factor-α, flagellin and interleukin-1β for 60 weeks. Then, the malignant phenotypes were evaluated using the MTS assay, Annexin V fluorescence assay, cell migration assay and sphere formation assay. The influence of P53 function on these phenotypes was assessed with a TP53 mutation model using the CRISPR/Cas9 system. A long-term inflammation model of LS174T cells was established for the first time with continuous inflammatory signalling. Chronic inflammation induced apoptosis and suppressed the proliferation and stemness of these cancer cells via the action of P53. It also enhanced the invasiveness of LS174T cells. Moreover, these phenotypic changes and changes in inflammatory signalling were recoverable after the removal of...Continue Reading

References

Jun 26, 2002·The British Journal of Surgery·A LeslieR J C Steele
Feb 16, 2008·Biochemical and Biophysical Research Communications·Mikayo AragakiMamoru Watanabe
Apr 27, 2010·Otolaryngology--head and Neck Surgery : Official Journal of American Academy of Otolaryngology-Head and Neck Surgery·Mariam DohadwalaMaie A St John
Feb 24, 2015·Gastroenterology·Loren LaineUNKNOWN SCENIC Guideline Development Panel
Apr 5, 2015·Digestive Diseases and Sciences·Masakazu Yashiro
Aug 2, 2016·Advanced Pharmaceutical Bulletin·Balal BrazvanHojjatollah Nozad Charoudeh
Apr 17, 2019·Molecular Cancer Research : MCR·Sho WatanabeMamoru Watanabe
Jun 4, 2019·Digestive and Liver Disease : Official Journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver·Makoto MutaguchiTakanori Kanai
Jun 26, 2020·Frontiers in Cell and Developmental Biology·Emilie d'AldebertNathalie Vergnolle
Feb 18, 2021·Journal of Crohn's & Colitis·Sho WatanabeKiichiro Tsuchiya
Feb 24, 2021·International Journal of Biological Macromolecules·Ezzatollah FathiBehnaz Valipour

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ELDA
ImageJ
extreme limiting dilution analysis ( ELDA )
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