PMID: 2120092Jul 9, 1990Paper

Influence of coculture with Sertoli cells on steroidogenesis in immature rat Leydig cells

Molecular and Cellular Endocrinology
G Verhoeven, J Cailleau

Abstract

The hypothesis has been advanced that Sertoli cells produce one or more follicle-stimulating hormone (FSH)-dependent paracrine factors which stimulate Leydig cell maturation and steroidogenesis. In an attempt to identify these factors we studied the effect of coculture with Sertoli cells on the steroidogenic capacity of immature Leydig cells. It is demonstrated that coculture, during a period of 6 days, markedly increases the capacity of the Leydig cells to secrete C21-steroids (progesterone, 17 alpha-hydroxyprogesterone, 20 alpha-hydroxypregn-4-en-3-one) and C19-steroids (testosterone, androst-4-ene-3,17-dione) in response to stimulation with luteinizing hormone (LH). Pretreatment of the cocultures on days 4, 5 and 6 with low concentrations of gonadotropins further enhances the steroidogenic response to LH. This pretreatment results in an overall increase in steroid output. At low concentrations of Sertoli cells and when short incubation times are used, pretreatment with FSH is clearly more effective than pretreatment with LH. Pretreatment with gonadotropins also results in a disproportionate increase in C19 output caused by increased conversion of C21 precursors into C19-steroids. This effect is also observed in Leydig cell m...Continue Reading

References

Apr 19, 1976·Biochemical and Biophysical Research Communications·M J Welsh, J P Wiebe
Jan 1, 1989·Annals of the New York Academy of Sciences·J M SaezP G Chatelain
Apr 1, 1985·Molecular and Cellular Endocrinology·G Verhoeven, J Cailleau
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Feb 1, 1987·Molecular and Cellular Endocrinology·G Verhoeven, J Cailleau
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Citations

Jul 1, 1992·Cell Biology and Toxicology·H LejeuneJ M Saez
Jun 23, 2001·Molecular and Cellular Endocrinology·R HabertJ M Saez
Apr 1, 1992·Baillière's Clinical Endocrinology and Metabolism·G Verhoeven
Jul 27, 1999·The Journal of Steroid Biochemistry and Molecular Biology·C Le RoyD Langlois
Mar 19, 2004·Biology of Reproduction·Varadaraj ChandrashekarAndrzej Bartke
Nov 3, 2005·Reproduction : the Official Journal of the Society for the Study of Fertility·Helen BainesFrancis J P Ebling
Aug 22, 2014·Biology of Reproduction·Colleen M CarneyJoan S Jorgensen
Sep 26, 2006·Molecular Reproduction and Development·C LécureuilF Guillou
Jan 1, 1992·Journal of Endocrinological Investigation·G GiordanoF Minuto
Aug 1, 1993·The Journal of Urology·D J Lamb

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