Influence of collagen source on fibrillar architecture and properties of vitrified collagen membranes

Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Shoumyo MajumdarJennifer Elisseeff

Abstract

Collagen vitrigel membranes are transparent biomaterials characterized by a densely organized, fibrillar nanostructure that show promise in the treatment of corneal injury and disease. In this study, the influence of different type I collagen sources and processing techniques, including acid-solubilized collagen from bovine dermis (Bov), pepsin-solubilized collagen from human fibroblast cell culture (HuCC), and ficin-solubilized collagen from recombinant human collagen expressed in tobacco leaves (rH), on the properties of the vitrigel membranes was evaluated. Postvitrification carbodiimide crosslinking (CX) was also carried out on the vitrigels from each collagen source, forming crosslinked counterparts BovXL, HuCCXL, and rHXL, respectively. Collagen membrane ultrastructure and biomaterial properties were found to rely heavily on both collagen source and crosslinking. Bov and HuCC samples showed a random fibrillar organization of collagen, whereas rH vitrigels showed remarkable regional fibril alignment. After CX, light transmission was enhanced in all groups. Denaturation temperatures after CX increased in all membranes, of which the highest increase was seen in rH (14.71°C), suggesting improved thermal stability of the colla...Continue Reading

References

Apr 1, 1996·Biomaterials·L H Olde DaminkJ Feijen
Jul 13, 2000·The New England Journal of Medicine·R J TsaiJ K Chen
Mar 27, 2001·The British Journal of Ophthalmology·A J Bron
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·E LeikinaS Leikin
Sep 26, 2003·Investigative Ophthalmology & Visual Science·Brian M StramerM Elizabeth Fini
Sep 24, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·A K LynnW Bonfield
Aug 22, 2006·Biomaterials·Heather M Powell, Steven T Boyce
Aug 31, 2007·Journal of Biomedical Materials Research. Part a·Frank EveraertsJan Feijen
Mar 14, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Winnette McIntosh AmbroseJennifer Elisseeff
Feb 22, 2011·Journal of Cataract and Refractive Surgery·Derek W DelMonte, Terry Kim
Mar 10, 2012·International Journal of Nanomedicine·Jui-Yang LaiTing-Chun Yu
Aug 28, 2012·Biomaterials·Xiomara Calderón-ColónMorgana M Trexler
Nov 15, 2012·Investigative Ophthalmology & Visual Science·Michael D DiVitoAllison Hubel
Dec 18, 2012·Tissue Engineering. Part a·Shane BrowneAbhay Pandit
Dec 21, 2012·Tissue Engineering. Part a·Oded ShoseyovFrida Grynspan
Feb 27, 2013·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Dermot CassidyMichael S Loughnan
Mar 15, 2013·Journal français d'ophtalmologie·E TrichetL Hoffart
May 3, 2013·Journal of Molecular Histology·Linan GuanPing Liu
May 30, 2013·Arquivos brasileiros de oftalmologia·Sérgio Kwitko

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Citations

May 31, 2018·Advanced Healthcare Materials·Rachelle N PalcheskoAdam W Feinberg
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Dec 7, 2016·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Giuseppe TronciDavid J Wood
Feb 25, 2021·ACS Biomaterials Science & Engineering·Yusuf B ArıkAndries D van der Meer
Jan 29, 2019·Biomaterials·Grissel Trujillo-de SantiagoNasim Annabi
Jan 20, 2021·ACS Biomaterials Science & Engineering·Xinhua LiuOuyang Yue

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