Influence of Cooling Rate on Growth of Bacillus cereus from Spore Inocula in Cooked Rice, Beans, Pasta, and Combination Products Containing Meat or Poultry

Journal of Food Protection
Vijay JunejaO Peter Snyder

Abstract

The objective of this study was to assess the ability of Bacillus cereus spores to germinate and grow in order to determine a safe cooling rate for cooked rice, beans, and pasta, rice-chicken (4:1), rice-chicken-vegetables (3:1:1), rice-beef (4:1), and rice-beef-vegetables (3:1:1). Samples were inoculated with a cocktail of four strains of heat-shocked (80°C for 10 min) B. cereus spores (NCTC 11143, 935A/74, Brad 1, and Mac 1) to obtain a final spore concentration of approximately 2 log CFU/g. Thereafter, samples were exponentially cooled through the temperature range of 54.5 to 7.2°C in 6, 9, 12, 15, 18, and 21 h. At the end of the cooling period, samples were removed and plated on mannitol egg yolk polymyxin agar. The plates were incubated at 30°C for 24 h. The net B. cereus growth from spores in beans was <1 log after 9 h of cooling, but the pathogen grew faster in rice and pasta. In combination products, the net growth was as follows: 3.05, 3.89, and 4.91 log CFU/g in rice-chicken; 3.49, 4.28, and 4.96 log CFU/g in rice-beef; 3.50, 4.20, and 5.32 CFU/g in rice-chicken-mixed vegetables; and 3.68, 4.44, and 5.25 CFU/g in rice-beef-mixed vegetables after 15, 18, and 21 h of cooling, respectively. This study suggests safe cooli...Continue Reading

References

Jan 1, 1994·Society for Applied Bacteriology Symposium Series·K Johnstone
Jan 1, 1994·Society for Applied Bacteriology Symposium Series·P E Granum
Jan 1, 1997·Journal of Applied Microbiology·C M BillonC Adair
Mar 15, 2001·International Journal of Food Microbiology·M Del TorreM L Stecchini
Feb 5, 2005·Applied and Environmental Microbiology·Luc M HornstraMarjon H J Wells-Bennik
Oct 11, 2005·Journal of Invertebrate Pathology·Stéphane PerchatMichel Gohar
Nov 23, 2005·Journal of Food Protection·Thomas J MontvilleDonald W Schaffner
Jan 13, 2006·Journal of Applied Microbiology·M Altayar, A D Sutherland
Mar 1, 2006·International Journal of Food Microbiology·Frédéric CarlinMonika Ehling-Schulz
Apr 25, 2006·Journal of Food Protection·Andreja RajkovicJohan Debevere
Nov 26, 2008·International Journal of Food Microbiology·Chandrakant AnkolekarRonald G Labbé
Nov 12, 2009·Journal of Food Protection·Chandrakant Ankolekar, Ronald G Labbé
Jan 5, 2011·Emerging Infectious Diseases·Elaine ScallanPatricia M Griffin
Apr 23, 2013·Food Chemistry·UNKNOWN Food Monitoring Group
Jul 1, 1981·Journal of Food Protection·Frank L BryanNorma Christopherson
Mar 1, 1989·Journal of Food Protection·Mas Angeles MossoMa Carmen DE LA Rosa

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.