Influence of copper (I) nicotinate complex and autophagy modulation on doxorubicin-induced cytotoxicity in HCC1806 breast cancer cells

Advances in Medical Sciences
Mohamed A Abdel-MohsenEman S El-Shafey

Abstract

Doxorubicin is regarded as the most therapeutic active agent available for triple-negative breast cancer (TNBC) treatment. However, the development of drug resistance and toxicity limits its effectiveness. Thus, developing novel strategies for TNBC treatment remains a significant challenge and doxorubicin-based combinations either by metal complexes (Copper I nicotinate complex) or with autophagy modulators could provide novel strategies and alternative strategies contributed to cancer cell death pathways, autophagy and apoptosis. The viability of HCC1806 TNBC cells and IC50 values of Doxorubicin (DOX), Torin-1 (TOR), Chloroquine (CQ) and Copper (I) nicotinate complex (CNC) were assessed by MTT assay. ELISA was used for detecting microtubule-associated protein 1 light chain 3 (LC3) level. Real time PCR was used to determine (NBR1) gene expression. Cell cycle analysis and quantitative detection of acid vesicular organelles (AVOs) was performed by flow cytometry. TOR and CQ were used as autophagy modulators for induction and suppression of autophagy, respectively. The half-maximal inhibition effect of TOR combination with DOX was revealed to the induction of autophagic cell death and apoptotic cell death. On the other hand, combi...Continue Reading

Citations

Sep 8, 2020·Journal of Zhejiang University. Science. B·Nan WenZheng-Gui Du
Nov 8, 2019·Anti-cancer Agents in Medicinal Chemistry·Eman S El-Shafey, Eslam S Elsherbiny
Apr 23, 2020·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Eman Salah El-Shafey, Eslam Samy Elsherbiny
Feb 23, 2021·Journal of Food Biochemistry·Ghada Mosad El SeedyEslam Samy Elsherbiny
Mar 12, 2021·Journal of Cellular Physiology·Yuping LuoMin Li

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