Influence of corticosteroids on myonuclear domain size in the rat diaphragm muscle

Journal of Applied Physiology
A Jeroen VerheulG C Sieck

Abstract

Skeletal muscle fibers are multinucleated. Each myonucleus regulates gene products and protein expression in only a restricted portion of the muscle fiber, the myonuclear domain (MND). In the rat diaphragm muscle (DIAm), corticosteroid (CoS) treatment causes atrophy of fibers containing myosin heavy chain (MHC): MHC2X and/or MHC2B. We hypothesized that DIAm fiber MND size is maintained during CoS-induced atrophy. Adult male rats received methylprednisolone for 11 days at 1 (CoS-Low, n = 8) or 8 mg x kg(-1) x day(-1) (CoS-High, n = 8). Age-matched (CTL-AgeM, n = 8), sham-operated (SHAM-AgeM, n = 8), and weight-matched (CTL-WtM, n = 8) animals served as controls. In single DIAm fibers, cross-sectional area (CSA), MND size, and MHC expression were determined. Fiber CSA and MND size were similar in CTL-AgeM and SHAM-AgeM groups. Only fibers containing MHCslow or MHC2A displayed smaller CSA in CTL-WtM than in CTL-AgeM and SHAM-AgeM groups, and MND size was reduced in all fibers. Thus fibers containing MHCslow and MHC2A maintain the number of myonuclei, whereas MHC2X or MHC2B fibers show loss of myonuclei during normal muscle growth. Both CoS groups displayed smaller CSA and MND size than CTL-AgeM and SHAM-AgeM groups. However, compa...Continue Reading

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Citations

Aug 25, 2009·The Journal of Physiological Sciences : JPS·Naomi E BrooksRobert S Hikida
Apr 9, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Susan D KranerMark M Rich
Nov 4, 2006·American Journal of Respiratory and Critical Care Medicine·Joseph M McClungScott K Powers
Nov 5, 2013·The Journal of Physiology·Gary C Sieck, Carlos B Mantilla
Sep 19, 2012·Respiratory Physiology & Neurobiology·Yasin B SevenGary C Sieck
Jul 19, 2011·Respiratory Physiology & Neurobiology·Carlos B Mantilla, Gary C Sieck
Jul 20, 2012·Wiley Interdisciplinary Reviews. Systems Biology and Medicine·Sarah M GreisingGary C Sieck
Mar 23, 2013·The FEBS Journal·Stefano SchiaffinoMarco Sandri
Jul 9, 2013·Respiratory Physiology & Neurobiology·Carlos B Mantilla, Gary C Sieck
Nov 20, 2016·Journal of Cellular Physiology·Jacob A RossJulien Ochala
Jul 5, 2005·Journal of Applied Physiology·Hui ZhongV Reggie Edgerton
Jan 18, 2006·Journal of Applied Physiology·Bharathi AravamudanGary C Sieck
Jun 9, 2007·Journal of Applied Physiology·Gary C SieckY S Prakash
Jan 12, 2008·Journal of Applied Physiology·Carlos B MantillaGary C Sieck
Feb 10, 2007·Journal of Applied Physiology·Scott K PowersJoseph M McClung

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