Influence of culture medium on smooth muscle cell differentiation from human bone marrow-derived mesenchymal stem cells.

Tissue Engineering. Part a
Zhaodi GongLaura E Niklason

Abstract

Human bone marrow-derived mesenchymal stem cells (hMSCs) represent an appealing source of smooth muscle cells (SMCs) for engineering small-diameter vascular grafts due to the limited availability and replicative capacity of somatic SMCs. However, lack of standardization of hMSC culture conditions has limited some progress in hMSC research. Because, at the moment, a chemically defined, serum-free medium without growth factors is not capable of amplifying hMSCs in vitro, the usage of serum (either human serum or fetal bovine serum [FBS]) continues in hMSC research. The emergence of commercial hMSCs and hMSC media opened a series of questions regarding the compatibility of commercial and homemade hMSCs and hMSC media. In this study, two types of commonly used FBS-containing hMSC media-MSCGM (containing 10% FBS) and MesenPro (containing 2% FBS), along with our homemade medium (low-glucose Dulbecco's modified Eagle's medium plus 10% selected lot FBS)-were compared in their ability to support SMC differentiation from hMSCs. The effects of FBS level, medium supplements (ascorbic acid, copper, etc.), and growth factors (transforming growth factor beta1) were also examined for their impact on SMC differentiation. It was discovered that ...Continue Reading

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Citations

Aug 7, 2013·Biochemical and Biophysical Research Communications·Xuejuan LiuYulin Li
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Olga TsigkouCraig Neville
Dec 6, 2011·Tissue Engineering. Part a·Joydeep BasuJohn W Ludlow
Feb 20, 2010·Tissue Engineering. Part B, Reviews·Jennifer H ChoiDavid L Kaplan
Nov 23, 2010·Cardiovascular Research·Krishna C VallabhaneniGabriele Eden
Nov 2, 2011·Cells, Tissues, Organs·Sumati Sundaram, Laura E Niklason
Jun 23, 2011·Journal of Visualized Experiments : JoVE·Angela H Huang, Laura E Niklason
May 5, 2012·Pulmonary Circulation·Amy L Firth, Jason X-J Yuan
Aug 7, 2010·Acta Biomaterialia·Corina VaterMaik Stiehler
Aug 31, 2010·Trends in Biotechnology·Joydeep Basu, John W Ludlow
Dec 3, 2014·Advanced Drug Delivery Reviews·Gerd KleinWilhelm K Aicher
Mar 20, 2015·Tissue Engineering. Part B, Reviews·Melanie L HartJan E Brinchmann
Feb 7, 2012·Biomaterials·Jeffrey T Krawiec, David A Vorp
Apr 10, 2012·Microvascular Research·Ulrich TiggesRichard Milner
Dec 17, 2014·Microvascular Research·Sebastian M GoerkeGünter Finkenzeller
Aug 15, 2014·Tissue Engineering. Part B, Reviews·Guenaelle BouetLaurence Vico
Feb 18, 2016·Journal of Biomedical Materials Research. Part a·Samaneh Moghadasi BoroujeniMasoud Soleimani
Dec 19, 2016·European Journal of Cell Biology·Sajjad SisakhtnezhadHassan Akrami
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Jun 28, 2018·Cellular and Molecular Life Sciences : CMLS·Ting ChenQingbo Xu

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Methods Mentioned

BETA
fluorescence-activated cell sorting
SMA
enzyme-linked immunosorbent 321
PCR
ELISA

Software Mentioned

MesenPro
AxioVision
Image J

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