Influence of dentine debris and organic tissue on the properties of sodium hypochlorite solutions

International Endodontic Journal
S TejadaM T Arias-Moliz

Abstract

To determine the free available chlorine of 2.5% sodium hypochlorite (NaOCl) alone and combined with 9% etidronic acid (HEDP) in the presence of inhibitors, organic tissue and organic tissue plus dentine debris; to evaluate the influence of dentine debris on the tissue-dissolving capacity of both NaOCl solutions; and to determine the antimicrobial action of these solutions when in contact with organic tissue and organic tissue plus dentine debris. The available chlorine of the solutions over time in the absence and presence of the inhibitors was measured using a titration method. The organic tissue dissolution by the solutions alone and in the presence of dentine powder was evaluated by weighing bovine tissue specimens before and after exposure to the solutions for 3 and 10 min. For the antimicrobial activity, biofilms of Enterococcus faecalis were exposed to the solutions for 3 min in the absence and presence of organic tissue and organic tissue + dentine debris. The biovolume and percentage of damaged membrane cells of the biofilm were measured by means of confocal microscopy and the live/dead technique. Nonparametric tests were used to determine statistical differences (P < 0.05). Both inhibitors consumed the free available ...Continue Reading

References

Apr 20, 2001·International Endodontic Journal·H K HaapasaloM P Haapasalo
Aug 24, 2002·International Endodontic Journal·I PortenierM Haapasalo
Oct 27, 2005·Journal of Endodontics·Matthias ZehnderTuomas Waltimo
Apr 25, 2006·Journal of Endodontics·Matthias Zehnder
Oct 22, 2008·Journal of Endodontics·José F Siqueira, Isabela N Rôças
Jul 2, 2009·Journal of Endodontics·Frank PaquéMatthias Zehnder
Feb 19, 2011·Journal of Endodontics·Pilar BacaCarmen María Ferrer-Luque
Jul 25, 2013·Journal of Endodontics·Iris Slutzky-GoldbergHagay Slutzky
Oct 22, 2013·Journal of Endodontics·Zhejun WangMarkus Haapasalo
Jun 5, 2014·International Endodontic Journal·T TartariC M Bramante
Jul 30, 2014·Journal of Endodontics·Markus HaapasaloMehrzad Khakpour
Oct 1, 2014·Journal of Endodontics·Maria Teresa Arias-MolizCarmen María Ferrer-Luque
Mar 10, 2016·Journal of Endodontics·Maria Teresa Arias-MolizPilar Baca

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