Influence of detergent additives on mobility of native and subviral rhinovirus particles in capillary electrophoresis

Electrophoresis
Leopold KremserErnst Kenndler

Abstract

The electrophoretic properties of two human rhinovirus (HRV) serotypes, HRV2 and HRV14, their subviral particles, and their capsid proteins were investigated by CE using borate buffer, pH 8.3, as BGE and three different detergents as additives. In addition, the influence of modification of the capsid with an amine reactive fluorescent dye, Cy3.5, on migration in the electric field was assessed. We found that the reproducibility of the electrophoretic results was decisively dependent on the presence of the detergents above their respective CMC. As compared to the strong ionic detergent SDS, the nonionic, mild detergent dodecylpoly(ethyleneglycol ether) (D-PEG) efficiently and reproducibly resolved both, native viruses as well as subviral particles. Most of the analytes behaved as expected except native HRV2; this serotype showed a dramatically higher anionic mobility in SDS than in D-PEG. Additionally, its mobility decreased when each positive charge contributed from a lysine at the capsid surface was substituted by four negative charges upon derivatization with Cy3.5. We discuss the possibility that this effect is caused by differences in number and in arrangement of exposed lysines in the two serotypes leading to differences i...Continue Reading

References

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Jul 16, 2004·Analytical Chemistry·Leopold KremserErnst Kenndler

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Citations

Dec 5, 2015·Electrophoresis·Virginie BettonvilleAnne-Catherine Servais
Aug 22, 2012·Journal of Pharmaceutical and Biomedical Analysis·Iuliana OitaYvan Vander Heyden
Nov 21, 2008·Journal of Pharmaceutical and Biomedical Analysis·Iuliana OitaYvan Vander Heyden
Apr 2, 2008·Journal of Chromatography. a·Maria Candida M MelladoAna Luísa Simplício
Nov 17, 2007·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Victor U WeissErnst Kenndler
Jul 12, 2007·Journal of Separation Science·Leopold KremserErnst Kenndler
Dec 23, 2008·Electrophoresis·Leopold KremserErnst Kenndler
Dec 7, 2007·Electrophoresis·Vladislav Dolník
Jun 26, 2008·Electrophoresis·Vratislav Kostal, Edgar A Arriaga
Oct 1, 2010·Electrophoresis·Hadewych HalewyckBart Rombaut
May 29, 2013·Electrophoresis·Victor U WeissGünter Allmaier
Mar 30, 2016·Analytical and Bioanalytical Chemistry·Victor U WeissGünter Allmaier
Mar 29, 2014·Journal of Virology·Shushan HarutyunyanDieter Blaas
Oct 27, 2017·Current Medicinal Chemistry·Agnieszka ChylewskaMariusz Makowski
Jan 7, 2009·Analytical Chemistry·Ewa Kłodzińska, Bogusław Buszewski

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