PMID: 2752022Aug 8, 1989Paper

Influence of dietary fatty acids on membrane fluidity and activation of rat platelets

Biochimica Et Biophysica Acta
J W HeemskerkG Hornstra

Abstract

The apparent steady-state fluorescence anisotropy of DPH- or TMA-DPH-labeled washed rat platelets is strongly affected by factors that also influence the turbidity by these platelet suspensions. Sonicated preparations from platelet lipids have a low turbidity and give anisotropy values which are hardly affected by the experimental conditions. We studied the effect of four high-fat diets on membrane fluidity, lipid composition and activation tendency of washed platelets. The diets contained 50 energy% of oils with different levels of saturated and (poly)unsaturated fatty acids. Only small diet-induced differences in DPH fluorescence anisotropy were found, which were comparable for intact platelets and platelet lipids. These differences were unrelated to the degree of saturation of the dietary fatty acids. Platelets from rats fed mainly saturated fatty acids differed significantly from other diet groups in a higher unsaturation degree of phospholipids and a lower cholesterol/phospholipid ratio, but this was not detected by DPH in terms of decreased anisotropy. These platelets aggregated less than other platelets in response to thrombin or collagen. The lower response to collagen persisted in indomethacin-treated platelets activat...Continue Reading

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Citations

Oct 1, 1994·Psychiatry Research·J K YaoJ L Peters
Oct 1, 1992·Molecular and Biochemical Parasitology·B H Ter KuileF R Opperdoes
Jun 27, 1990·Biochimica Et Biophysica Acta·M A FeijgeG Hornstra
Apr 1, 1993·Cephalalgia : an International Journal of Headache·M D CotrimT dos R de Macedo
Feb 16, 2006·Molecular and Cellular Biochemistry·Jayadev RajuRanjana P Bird
Jan 1, 1991·Life Sciences·K H Le Quan SangM A Devynck
Jan 1, 1993·Life Sciences·N VlasicM B Stemerman
Mar 1, 1991·Chemistry and Physics of Lipids·P K Kinnunen
Jun 3, 1991·Biochimica Et Biophysica Acta·R C VossenR F Zwaal
Sep 1, 1993·Prostaglandins, Leukotrienes, and Essential Fatty Acids·E Malle, G M Kostner

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