PMID: 8944449Oct 1, 1996Paper

Influence of different collagenase solvents and timing of their delivery on porcine islet isolation

The British Journal of Surgery
S A WhiteN J London

Abstract

Methods to minimize the effect of cold ischaemia on porcine islet isolation were investigated. Forty pancreata were randomized to intraductal collagenase delivery in University of Wisconsin solution (UW) or Hanks balanced salt solution (HBSS) (control) both before and after 65 min of cold pancreas storage. Collagenase was also administered in a specially designed cold storage solution (University of Leicester solution, ULEIC), before cold storage. Median islet yield was significantly greater if the pancreas was distended with collagenase in either UW (21524 islet equivalents (IEQ) per g) or ULEIC (19814 IEQ/g) before cold storage, compared with that after distension with HBSS (6924 IEQ/g) following cold storage (P < 0.05). Islet fragmentation, islet purification and glucose-stimulated insulin release were not significantly different after collagenase delivery in either UW or ULEIC compared with those after administration in HBSS. It is concluded that porcine islet yields can be improved significantly by intraductal collagenase administration in either UW or ULEIC immediately after excision of the pancreas.

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Citations

Jun 26, 2001·Human Immunology·C J SwansonT Mohanakumar
Mar 17, 2001·Diabetic Medicine : a Journal of the British Diabetic Association·S A WhiteM L Nicholson
Jan 16, 2007·Transplantation·Tatsuya KinJonathan R T Lakey
Jun 22, 2005·Treatments in Endocrinology·Daniel M RidgwayRachel M Kimber
May 25, 2010·HPB : the Official Journal of the International Hepato Pancreato Biliary Association·Seok L OngAshley R Dennison
Nov 24, 2005·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·J AvilaJ Oberholzer
Dec 18, 2009·Clinical Transplantation·Dan RidgwaySteve White
May 2, 2008·Xenotransplantation·Sangeetha Prabhakaran, Bernhard J Hering
Sep 6, 2000·Cell Transplantation·C TosoP Morel

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