Influence of different luminols on the characteristics of the chemiluminescence reaction in human neutrophils

Journal of Bioluminescence and Chemiluminescence
H LundqvistC Dahlgren

Abstract

In search for a luminol with very high output of light, 20 different luminol samples were tested for their ability to enhance the chemiluminescence reaction in phorbol myristate acetate activated human neutrophils. We found that the majority of luminols tested (17 samples) gave almost the same light output from neutrophils, and that the major part of the activity was from an intracellular origin. Owing to the fact that three isoluminol samples were unable to monitor respiratory burst activity taking place intracellularly, a very low level of chemiluminescence was obtained with these samples. Their light output was, however, greatly increased when horseradish peroxidase or myeloperoxidase was added, showing that the light-generating reaction with isoluminol as well as with luminol is peroxidase-dependent. The fact that isoluminol could also use myeloperoxidase as amplifying peroxidase, suggests that that the lack of measurable intracellular activity in the presence of isoluminol is somehow related to a limited or restricted diffusion of the molecule to intracellular sites. The isoluminol system constitutes a sensitive system for measuring release of oxygen metabolites from phagocytic cells.

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Citations

Dec 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·H Lundqvist, C Dahlgren
Jun 25, 2008·The British Journal of Nutrition·Siegfried KnasmüllerKarl-Heinz Wagner
Aug 13, 2011·International Journal of Laboratory Hematology·M RájeckýM Cíž
Oct 28, 2006·Veterinary Immunology and Immunopathology·Manuela RinaldiDouglas D Bannerman
Sep 18, 2012·Free Radical Biology & Medicine·Ghassan J MaghzalVincent Jaquet
Aug 3, 2001·Journal of Biomedical Materials Research·H NygrenL Oster
Jul 9, 1999·Journal of Biomedical Materials Research·H NygrenI Lundström
Jul 17, 2013·The Journal of Biological Chemistry·Anthony M FranchiniJames R Drake
Jun 1, 2016·Journal of Biophotonics·Cláudio Daniel CerdeiraFelipe Fornias Sperandio
May 1, 2000·Alternatives to Laboratory Animals : ATLA·H NygrenN Yahyapour
Jul 17, 1998·Analytical Chemistry·A W Griffith, J M Cooper

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