Influence of diluents, media, and membrane filters on detection fo injured waterborne coliform bacteria.

Applied and Environmental Microbiology
G A McFetersM W LeChevallier

Abstract

Pure cultures of Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, and Citrobacter freundii were injured ( greater than 90%) in water from a dead-end section of the Bozeman, Montana, distribution system. The effects of the following laboratory variables on the enumeration efficiency of injured and undamaged control cells were examined: (i) diluent composition, temperature, and time of exposure; (ii) media, using various formulations employed in enumerating gram-negative bacteria; and (iii) surface pore morphology of membrane filters. The addition of peptone or milk solids to diluents and low temperature (4 degrees C) maximized the recovery of injured cells, but had little effect on undamaged cells. Control cells were recovered with high efficiencies on most media tested, but recoveries of injured cells ranged from 0 to near 100%. Most of the media commonly used in water analysis recovered less than 30% of injured cells. This was explained in part by the sensitivity of injured bacteria to deoxycholate concentrations greater than 0.01%, whereas control cells were unaffected by 0.1%. Membrane filter surface pore morphology (at 35 degrees C) had a negligible effect on total coliform recoveries. Recommendations are ma...Continue Reading

References

Jul 1, 1977·Applied and Environmental Microbiology·D G StuartJ E Schillinger
Jul 1, 1977·Applied and Environmental Microbiology·R S Tobin, B J Dutka
Aug 1, 1977·Canadian Journal of Microbiology·A Hurst
Mar 1, 1979·Applied and Environmental Microbiology·A K Camper, G A McFeters
May 1, 1976·Canadian Journal of Microbiology·B J Dutkaand, S E Tobin
Oct 1, 1976·Applied and Environmental Microbiology·S D Lin
Apr 1, 1975·Applied Microbiology·R E RoseW Litsky
Oct 1, 1975·Applied Microbiology·K J SladekF W Dawson
Mar 1, 1974·The Journal of Applied Bacteriology·A W Hoadley, C M Cheng
Apr 1, 1973·Applied Microbiology·B Ray, M L Speck
Aug 1, 1974·Applied Microbiology·J R Braswell, A W Hoadley
Nov 1, 1969·Applied Microbiology·W A Weiler, S E Hartsell
Jan 1, 1971·Applied Microbiology·D L ScheusnerM L Speck
Aug 1, 1980·Applied and Environmental Microbiology·S K ZaskeG A McFeters
Jan 1, 1957·Applied Microbiology·R P STRAKA, J L STOKES
Mar 1, 1977·Applied and Environmental Microbiology·G K BissonnetteD G Stuart
Apr 1, 1927·Journal of Bacteriology·C E Winslow, O R Brooke
May 1, 1932·Journal of Bacteriology·C T Butterfield

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Citations

Feb 13, 2001·Journal of Applied Microbiology·H Perry-O'KeefeJ J Hyldig-Nielsen
Feb 1, 1985·Bulletin of Environmental Contamination and Toxicology·G CenciG Caldini
Apr 7, 2010·Applied and Environmental Microbiology·Shahar RotemAmram Mor
Sep 1, 1987·The Journal of Applied Bacteriology·K P Flint
Jun 30, 2010·FEMS Immunology and Medical Microbiology·Andreana MarinoGiuseppe Bisignano
May 1, 2001·Letters in Applied Microbiology·S MassaC Mammina
Apr 9, 2019·Journal of the Royal Society, Interface·Mara Otero FernandezJonathan P Reid
Nov 14, 2018·Critical Reviews in Food Science and Nutrition·Sarah L JonesKristen E Gibson
Jan 5, 2002·Journal of Microbiological Methods·Annie RompréPatrick Laurent
Jan 4, 2001·International Journal of Food Microbiology·M S Chae, H Schraft
Nov 1, 1984·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Dec 1, 1987·Applied and Environmental Microbiology·G R FinchD W Smith
Aug 1, 1984·Applied and Environmental Microbiology·M J DomekG A McFeters
Aug 1, 1984·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Oct 1, 1983·Applied and Environmental Microbiology·M Chen, P J Hickey
Aug 1, 1985·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Dec 1, 1985·Applied and Environmental Microbiology·A K CamperG A McFeters
Aug 1, 1985·Applied and Environmental Microbiology·A SinghG A McFeters
Nov 6, 2004·Journal of Clinical Microbiology·Michael R Barer, Gregg Bogosian
Jan 1, 1984·Applied and Environmental Microbiology·G A BurlingameW O Pipes
Feb 1, 1983·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Jan 1, 1994·Journal of Microbiological Methods·F P Yu, G A McFeters
Mar 1, 1983·Applied and Environmental Microbiology·M W LeChevallierG A McFeters
Nov 1, 1988·Applied and Environmental Microbiology·K R Gurijala, M Alexander
Sep 1, 1987·Applied and Environmental Microbiology·D E WilliamsL J Swango
Jul 1, 1994·Applied and Environmental Microbiology·F P Yu, G A McFeters
Jan 1, 1986·Applied and Environmental Microbiology·G A McFetersM W LeChevallier
Apr 1, 1986·Applied and Environmental Microbiology·A Singh, G A McFeters
Apr 1, 1986·Applied and Environmental Microbiology·R K Alico, M F Dragonjac
Feb 1, 1986·Applied and Environmental Microbiology·M J BlaserJ C Hoff
Feb 1, 1990·Applied and Environmental Microbiology·A SinghG A McFeters

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