Influence of dimethyl sulfoxide on extracellular enzyme production by Pleurotus ostreatus

Biotechnology Letters
Vishal ShahRichard Gross

Abstract

Dimethyl sulfoxide (DMSO) is commonly used as a co-solvent to dissolve poorly water-soluble biologically active agents to assess their biological activities such as for enzyme induction. The question addressed was whether DMSO can be assumed to be an inert co-solvent. The influence of DMSO on the production of extracellular enzymes by Pleurotus ostreatus was investigated. DMSO functioned as either an inducer or a repressor, depending on the enzyme studied. The production of laccase and endo-1,4-beta-xylanase increased by 29 and 250%, respectively, in presence of DMSO. However, DMSO repressed the activities of manganese peroxidase, beta-glucosidase, beta-xylanase, and endo-1,4-beta-glucanase by 30, 33, 99 and 16%, respectively. These results raise concerns about the interpretation of bioactivity measurements when DMSO is assumed to function as an inert co-solvent to solubilize water-insoluble molecules.

References

Jul 27, 1999·Bioscience, Biotechnology, and Biochemistry·J TanakaY Yamada
Apr 3, 2003·FEMS Microbiology Letters·Petr Baldrian, Jirí Gabriel
Jun 2, 2005·Biotechnology Letters·Pablo Domínguez de MaríaJosé V Sinisterra

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