Influence of Dimethyl Sulfoxide on the Low-Temperature Behavior of Cholesterol-Loaded Palmitoyl-oleyl-phosphatidylcholine Membranes

The Journal of Physical Chemistry. B
Beatrice GironiPaola Sassi

Abstract

The properties of lipid membranes at low temperature are important for a number of biomedical and biotechnological applications, and the success of these applications depends on understanding the effects of temperature changes on intermolecular lipid-lipid and lipid-water interactions. Here, we use Fourier transform infrared spectroscopy to study lipid suspensions in water/dimethyl sulfoxide (DMSO) solutions in the -60 to 30 °C range. DMSO is a cryopreservative agent of cellular systems, and its action is largely related to its interaction with the lipid membrane, especially in the low-temperature regime. In the present work, we analyze the effects of solvent composition on the structural and thermotropic properties of cholesterol (chol)-loaded liposomes of palmitoyl-oleylphosphatidylcholine (POPC) because POPC/chol liposomes are suitable models of the plasmatic membrane. To this extent, we compare the properties of lipid vesicles suspended in water and water/DMSO solution at 0.10 DMSO mole fraction and we observe that the gel phase of the membrane has an increased thermal stability on DMSO addition. We estimate that the amount of unfrozen water at T = -60 °C is much reduced by the presence of DMSO, both in the gel- and the liq...Continue Reading

References

Aug 1, 1968·Canadian Journal of Biochemistry·N H TattrieR Cyr
Dec 28, 1968·Nature·D H Rasmussen, A P MacKenzie
Jun 5, 1997·Nature·K Simons, E Ikonen
Mar 29, 2003·Biochemical Pharmacology·Nuno C SantosCarlota Saldanha
Apr 30, 2003·Biophysical Journal·Andrey FilippovGöran Lindblom
May 14, 2004·Annual Review of Biophysics and Biomolecular Structure·Kai Simons, Winchil L C Vaz
May 28, 2005·The Journal of Chemical Physics·J-B BrubachP Roy
Apr 11, 2006·The Journal of Membrane Biology·Norbert KucerkaJohn F Nagle
Feb 3, 2007·American Journal of Hematology·David BerzPeter J Quesenberry
Apr 10, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Carolyn M McQuawNicholas Winograd
Sep 9, 2008·Biochimica Et Biophysica Acta·Peter J Quinn, Claude Wolf
Oct 28, 2008·Biochimica Et Biophysica Acta·Félix M GoñiJenifer L Thewalt
Nov 11, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Dong-Kuk LeeAyyalusamy Ramamoorthy
Feb 24, 2009·Biochimica Et Biophysica Acta·Saravana K BalasubramanianJohn C Bischof
Oct 27, 2012·Biochimica Et Biophysica Acta·Ruthven N A H Lewis, Ronald N McElhaney
Mar 14, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Martin R KrauseSteven L Regen
Jul 25, 2014·Nature Communications·Yong-Sang RyuSin-Doo Lee
Dec 11, 2014·Physical Review Letters·Laura ToppoziniMaikel C Rheinstädter
Aug 19, 2015·Biophysical Chemistry·A GiugliarelliA Morresi
Apr 9, 2016·Chemistry and Physics of Lipids·Drew MarquardtJohn Katsaras
Nov 5, 2016·Biochimica Et Biophysica Acta·Maria RicciPaola Sassi
Nov 5, 2017·Biochimica Et Biophysica Acta. Biomembranes·Bulat SydykovWillem F Wolkers
Mar 3, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·E N KozlovskayaA A Kamnev

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Citations

Oct 16, 2019·Physical Chemistry Chemical Physics : PCCP·Beatrice GironiPaola Sassi
Dec 12, 2019·The Journal of Chemical Physics·Pratibha Kumari, Hemant K Kashyap
Sep 10, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Giuseppe M PaternòGuglielmo Lanzani
Aug 27, 2019·Biochimica Et Biophysica Acta. Biomembranes·Beatrice GironiPaola Sassi

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