Influence of disordered packing pattern on elastic modulus of single-stranded DNA film on substrate

Biomechanics and Modeling in Mechanobiology
W L MengZ Q Tan

Abstract

Determining mechanical properties of single-stranded DNA film grafted on gold surface is critical for analysis and design of DNA-microcantilever biosensors. However, it remains an open issue to quantify the relations among the disordered packing patterns of DNA chains, the mechanical properties of DNA film and the resultant biodetection signals. In this paper, first, the bending experiment of microcantilever is carried out to provide the basic data for a refined multi-scale model of microcantilever deflection induced by ssDNA immobilization. In the model, the complicated interactions in DNA film (consisting of DNA, water molecules and salt ions) are simplified as effective interactions among coarse-grained soft cylinders, which can reveal the varieties of DNA structure in the circumstances of different lengths and salt concentrations; Ohshima's distribution of net charge density is employed to incorporate compositional variations of salt ions along the thickness direction into the Strey's mesoscopic empirical potential on molecular interactions in DNA solutions, and the related model parameters for ssDNA film on substrate are obtained from the curve fitting with our microcantilever bending experiment. Second, the effect of nano...Continue Reading

References

Apr 15, 2000·Science·J FritzJ K Gimzewski
Jul 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Rachel McKendryChristoph Gerber
Apr 23, 2003·Biosensors & Bioelectronics·Fei LiuZhong can Ou-Yang
May 24, 2005·Biophysical Journal·A HalperinE B Zhulina
Oct 4, 2005·Methods : a Companion to Methods in Enzymology·Karolyn M Hansen, Thomas Thundat
Oct 6, 2005·Biosensors & Bioelectronics·Guillaume LegayAlain Dereux
Dec 28, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Jeanne C StachowiakArun Majumdar
Apr 11, 2008·Journal of Biomedicine & Biotechnology·T PellegrinoW J Parak
Aug 22, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·M Arroyo-HernándezJ L Costa-Krämer
Jan 19, 2010·Nanotechnology·Michel GodinPeter Grutter
Mar 17, 2010·Biomechanics and Modeling in Mechanobiology·V K GuptaCharles D Eggleton
Jun 8, 2010·Biomechanics and Modeling in Mechanobiology·N H Zhang, J Y Shan
Jun 17, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ling HuangMarcel Utz
Oct 20, 2010·Computer Physics Communications·J Ambia-GarridoB Montgomery Pettitt
Jan 15, 2011·Acta Biomaterialia·Azadeh SeidiAli Khademhosseini
Nov 16, 2012·Chemical Society Reviews·Javier TamayoMontserrat Calleja
Nov 20, 2012·Biomechanics and Modeling in Mechanobiology·Mehdi KhoshgoftarCorrinus C van Donkelaar
Aug 26, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Carmen M DomínguezMontserrat Calleja

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.