PMID: 7130639Nov 1, 1982

Influence of disulfide-reducing agents on fractionation of the chromatin complex by endogenous nucleases and deoxyribonuclease I in aging mice

Journal of Gerontology
S Tas, R L Walford

Abstract

Age-related alterations in chromatin were evaluated by a fractionation procedure involving limited digestion of liver cell nuclei from young adult and old mice with endogenous nucleases and deoxyribonuclease I. The results suggest that in the chromatin the bulk of the nuclear deoxyribonucleic acid (DNA) may be folded or compacted into domains or loops and associated at a few points with a nuclear protein skeleton structure represented by the final pellet fraction which is resistant to deoxyribonuclease I. Disulfide bonds appear to play a role in the linkage of regions of DNA with this nuclear protein skeleton structure. The amount of DNA that could be released from the pellet fraction by disulfide-reducing agents was significantly greater with old than with young adult mice. In addition, the amount of chromatin material released into the first soluble fraction decreased and that in the second soluble fraction increased with age. Treatment of the nuclei with disulfide-reducing agents did not correct this particular age-related change. Previous results had shown a similar alteration with age of chromatin subjected to digestion by micrococcal nuclease. The combined results suggest the existence of a supranucleosomal alteration in ...Continue Reading

Citations

Feb 1, 1991·Journal of Cellular Physiology·R T Dell'Orco, L E Anderson
Dec 1, 1984·Mechanisms of Ageing and Development·Z A Medvedev
Oct 31, 1984·Mechanisms of Ageing and Development·M K Thakur
Jan 1, 1990·Experimental Gerontology·R S Sohal, R G Allen
Mar 1, 1996·The International Journal of Biochemistry & Cell Biology·Z KiliańskaW M Krajewska
Jun 10, 2010·Free Radical Research·Jelena MarkovicFederico V Pallardó
Apr 14, 2009·Free Radical Biology & Medicine·Michael J Hitchler, Frederick E Domann
Jun 15, 2007·Proceedings of the National Academy of Sciences of the United States of America·Rowena R RamosJoseph Bass

Related Concepts

Senility
Cell Nucleus
Chromatin
Alkaline DNase
Disulfides
DNA, Double-Stranded
Liver
Oxidation-Reduction

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