Influence of DNA adsorption and DNA/cationic surfactant coadsorption on the interaction forces between hydrophobic surfaces

Langmuir : the ACS Journal of Surfaces and Colloids
Alan D BraemBjörn Lindman

Abstract

The forces between hydrophobic surfaces with physisorbed DNA are markedly and irreversibly altered by exposure to DNA/cetyltrimethylammonium bromide (CTAB) mixtures. In this colloidal probe atomic force microscopy study of the interactions between a hydrophobic polystyrene particle and an octadecyltrimethylethoxysilane-modified mica surface in sodium bromide solutions, we measure distinct changes in colloidal forces depending on the existence and state of an adsorbed layer of DNA or CTAB-DNA complexes. For bare hydrophobic surfaces, a monotonically attractive approach curve and very large adhesion are observed. When DNA is adsorbed at low bulk concentrations, a long-range repulsive force dominates the approach, but on retraction some adhesion persists and DNA bridging is clearly observed. When the DNA solution is replaced with a CTAB-DNA mixture at relative low CTAB concentration, the length scale of the repulsive force decreases, the adhesion due to hydrophobic interactions greatly decreases, and bridging events disappear. Finally, when the surface is rinsed with NaBr solution, the length scale of the repulsive interaction increases modestly, and only a very tiny adhesion remains. These pronounced changes in the force behavior...Continue Reading

References

Sep 26, 1997·Nature·I M Verma, N Somia
Feb 19, 1996·Physical Review Letters·R M OverneyG Overney
Aug 28, 1999·Molecular Biotechnology·N S Templeton, D D Lasic
Aug 15, 2000·Journal of Chromatography. B, Biomedical Sciences and Applications·B M SteelsC A Haynes
Apr 20, 2001·Advanced Drug Delivery Reviews·M C Pedroso de LimaN Düzgüneş
Jun 24, 2003·Advances in Colloid and Interface Science·Per M ClaessonOrlando J Rojas
Nov 18, 2005·Journal of Colloid and Interface Science·Naoyuki IshidaKo Higashitani

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Citations

Jul 5, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·E GruesoP Lopez-Cornejo
Feb 3, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Chien-Wen ChangSung Wan Kim
May 30, 2020·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Laura L OsornoMark E Byrne
Apr 6, 2005·Langmuir : the ACS Journal of Surfaces and Colloids·Marité CárdenasBjörn Lindman
Jun 13, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Marité CárdenasThomas Arnebrant
Jan 29, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·J ZhangJ Penfold
May 2, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Kai Loon ChenMenachem Elimelech

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