Influence of droplet size, capillary-cone distance and selected instrumental parameters for the analysis of noncovalent protein-ligand complexes by nano-electrospray ionization mass spectrometry

Journal of Mass Spectrometry : JMS
Kurt BenkestockJohan Roeraade

Abstract

It has been suggested in the literature that nano-electrospray ionization (nano-ESI) mass spectrometry better reflects the equilibrium between complex and free protein in solution than pneumatically assisted electrospray ionization (ESI) in noncovalent interaction studies. However, no systematic studies of the effects of ionization conditions have been performed to support this statement. In the present work, different instrumental and sample-derived parameters affecting the stability of noncovalent complexes during analysis by nano-ESI were investigated. In general, increased values of parameters such as drying gas flow-rate, ion-source temperature, capillary tip voltage and buffer concentration lead to a dissociation of ribonuclease A (RNAse)-cytidine 2'-monophosphate (CMP) and cytidine 5'-triphosphate (CTP) complexes. The size of the electrosprayed droplets was shown to be an important issue. Increasing the capillary to cone distance yielded an increased complex to free protein ratio when a hydrophilic ligand was present and the reverse effect was obtained with a hydrophobic ligand. Important in this regard is the degree of sampling of ions originating from late-generation residue droplets, that is, ions present in the dropl...Continue Reading

Citations

Sep 29, 2011·Journal of the American Society for Mass Spectrometry·Harry J SterlingEvan R Williams
Dec 14, 2011·Journal of the American Society for Mass Spectrometry·Harry J SterlingEvan R Williams
Sep 6, 2012·Journal of the American Society for Mass Spectrometry·Tawnya G Flick, Evan R Williams
Jun 21, 2008·Journal of the American Society for Mass Spectrometry·Sheng YinJoseph A Loo
Aug 18, 2009·Journal of the American Society for Mass Spectrometry·Harry J Sterling, Evan R Williams
Jun 29, 2010·Journal of the American Society for Mass Spectrometry·Eric MartineauAhmed M Aman
Aug 3, 2010·Journal of the American Society for Mass Spectrometry·Harry J SterlingEvan R Williams
Jul 4, 2006·Nature Reviews. Drug Discovery·Steven A Hofstadler, Kristin A Sannes-Lowery
Mar 15, 2011·Physical Chemistry Chemical Physics : PCCP·Harry J SterlingEvan R Williams
Aug 20, 2014·Journal of the American Society for Mass Spectrometry·Rachel R Ogorzalek LooJoseph A Loo
Mar 16, 2005·Rapid Communications in Mass Spectrometry : RCM·Gustav SundqvistJohan Roeraade
Oct 9, 2007·Journal of Peptide Science : an Official Publication of the European Peptide Society·Andreas MarquardtMichael Przybylski
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Apr 24, 2012·Journal of Peptide Science : an Official Publication of the European Peptide Society·Madalina MafteiMichael Przybylski
Nov 1, 2005·Journal of Pharmaceutical and Biomedical Analysis·Gejing Deng, Gautam Sanyal
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Jul 12, 2018·Rapid Communications in Mass Spectrometry : RCM·Xiaojun LiPeng Li
Oct 4, 2018·Journal of Mass Spectrometry : JMS·Xinyao JingCarolyn J Cassady
Nov 9, 2004·Journal of Mass Spectrometry : JMS
Sep 8, 2021·The Journal of Physical Chemistry. a·Tina HeraviDavid V Dearden
Feb 10, 2007·Analytical Chemistry·Mark C Kuprowski, Lars Konermann

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