Influence of earthworm activity on gene transfer from Pseudomonas fluorescens to indigenous soil bacteria.

Applied and Environmental Microbiology
L L DaaneM J Sadowsky

Abstract

We have developed a model system to assess the influence of earthworm activity on the transfer of plasmid pJP4 from an inoculated donor bacterium, Pseudomonas fluorescens C5t (pJP4), to indigenous soil microorganisms. Three different earthworm species (Lumbricus terrestris, Lumbricus rubellus, and Aporrectodea trapezoides), each with unique burrowing, casting, and feeding behaviors, were evaluated. Soil columns were inoculated on the surface with 10(8) cells per g of soil of the donor bacterium, and after a 2-week incubation period, donor, transconjugant, and total bacteria were enumerated at 5-cm-depth intervals. Transconjugants were confirmed by use of colony hybridization with a mer gene probe. In situ gene transfer of plasmid pJP4 from P. fluorescens C5t to indigenous soil bacteria was detected in all inoculated microcosms. In the absence of earthworms, the depth of recovery was limited to the top 5 cm of the column, with approximately 10(3) transconjugants per g of soil. However, the total number of transconjugants recovered from soil was significantly greater in microcosms containing either L. rubellus or A. trapezoides, with levels reaching about 10(5) CFU/g of soil. In addition, earthworms distributed donor and transcon...Continue Reading

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Citations

Nov 1, 2002·FEMS Microbiology Ecology·Jan Dirk van Elsas, Mark J Bailey
Jan 5, 2000·Applied and Environmental Microbiology·D T NewbyI L Pepper
Jul 27, 2006·FEMS Microbiology Ecology·Anne KersantéFrançoise Binet
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Apr 5, 2013·FEMS Microbiology Reviews·Michiel VosGeorge A Kowalchuk
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Mar 1, 2003·The New Phytologist·Jan Dirk Van ElsasMark J Bailey
Jul 12, 2001·Research in Microbiology·V C Nwosu
Aug 12, 2009·Ecotoxicology and Environmental Safety·Beatriz MorenoEmilio Benitez

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