Influence of electrical parameters in the iontophoretic delivery of a small peptide: in vitro studies using arginine-vasopressin as a model peptide

Il Farmaco
Vinod Nair, Ramesh Panchagnula

Abstract

The present investigation was carried out to understand the influence of electrical parameters on iontophoretic transport of a small peptide like arginine-vasopressin (AVP). In vitro studies using rat skin were conducted to assess the effect of different current densities (CDs), durations, duty cycles and alternating polarity on vasopressin permeation. HPLC was used for ensuring electrochemical stability of the peptide whereas FT-IR and TGA were used to understand the biophysical changes caused in skin due to passage of current. Application of CD > 0.75 mA/cm(2) was found to compromise skin barrier integrity as well as electrochemical stability. Periodic current did not show any significant difference in permeation compared to continuous current. Alternating polarity was useful in reducing pH shift however, was less efficient compared to continuous direct current. FT-IR and TGA studies showed that skin hydration increased as a function of CD and duration and all the results could be explained on the basis of increased skin hydration.

References

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May 14, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Vinod Nair, Ramesh Panchagnula
May 14, 2003·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Vinod B Nair, Ramesh Panchagnula

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Citations

Jun 29, 2012·Archives of Dermatological Research·Madoka KageFumie Hashimoto
May 22, 1992·Biochimica Et Biophysica Acta·C K WangH C Cheung
Aug 1, 2011·Drug Delivery and Translational Research·Sanyog JainNitin K Swarnakar
Oct 1, 2008·Pharmaceutical Development and Technology·Maansi G Kumar, Senshang Lin
Jan 29, 2014·Pharmaceutical Development and Technology·Niketkumar PatelSenshang Lin
Feb 26, 2015·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Sylvain KotzkiJean-Luc Cracowski
Jul 11, 2013·The Journal of Pharmacology and Experimental Therapeutics·Sylvain KotzkiJean-Luc Cracowski

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