PMID: 20626143Jul 16, 2010Paper

Influence of electroacupuncture on myocardial NO and NOS and intracellular Ca2+ contents in myocardial ischemia-reperfusion injury rats

Zhen ci yan jiu = Acupuncture research
Chao WangJie Yan

Abstract

To observe the effect of electroacupuncture (EA) on contents of myocardial nitric oxide (NO), nitric oxide synthase (NOS), and intracellular Ca2+ levels in experimental myocardial ischemia-reperfusion injury (MI/RI) rats, so as to provide some evidence for acupuncture treatment of cardiovascular disorders and for the correlation between the acupoints and the internal organs. Fifty male Wistar rats were randomly divided into sham-operation (sham), model, Neiguan (PC 6), Lieque (LU 7), and Hegu (LI 4) groups (n = 10/group). MI/RI model was established by occlusion of the descending anterior branch of the left coronary artery for 40 min and reperfusion for 60 min. EA (30 Hz/100 Hz, 2-4 mA) was applied to the bilateral PC 6, LU 7 and LI 4 for 20 min. The myocardial tissue of the ischemic area was taken, homogenated and centrifuged, separated for assaying NO and NOS activity by using spectrophotometer and their contents with nitrate reductase colorimetric assay. Another part of the myocardium tissue was processed to be cell suspension (added with Fluo-3/AM dye) for assaying intracellular free [Ca2+]i concentration under the laser confocal microscope. Compared with sham group, myocardial NO and NOS contents of model group decreased m...Continue Reading

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