Influence of environmental cues on transcriptional regulation of foo and clp coding for F165(1) and CS31A adhesins in Escherichia coli

Research in Microbiology
C CrostChristine Martin

Abstract

F165(1) (foo) and CS31A (clp) are bacterial adhesins synthesized by Escherichia coli strains associated with diarrhea and septicemia in piglets and calves. They belong to the P-regulatory family and as such are subject to a phase variation control mediated by Lrp (leucine responsive regulatory protein) and regulators homologous to PapI. Analysis of expression of transcriptional fusions between the fooB or fooI promoters and lacZ showed that Lrp is an activator of foo and fooI transcription, whereas it represses clp transcription. Furthermore, foo phase variation leads to a large majority of phase-ON cells, whereas clp phase variation leads to a majority of phase-OFF cells. We compared the influence of several environmental cues on foo and clp expression, with special attention to the effects of leucine and alanine known to be mediated by Lrp. Inhibition or significant repression of foo and clp transcription was observed at low temperature, in LB medium, and in the presence of glucose, alanine, or leucine. Glucose repression of foo but not of clp was totally relieved by addition of cAMP. Osmolarity and pH had little effect. Alanine but not leucine, and LB medium inhibited foo and clp phase variation, locking cells in the OFF pha...Continue Reading

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Citations

Dec 4, 2004·Journal of Bacteriology·Frédéric BerthiaumeJosée Harel
Sep 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Josep Casadesús, David Low
Dec 11, 2008·Tropical Animal Health and Production·Reza Ghanbarpour, Eric Oswald
Feb 28, 2009·Advances in Applied Microbiology·Wolfgang Schumann
Dec 21, 2010·Journal of Bacteriology·Benjamin R Hart, Robert M Blumenthal
Jul 15, 2011·Molecular Microbiology·Richard GravelineJosée Harel
Apr 1, 2007·EcoSal Plus·Ian Blomfield, Marjan van der Woude

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