PMID: 22567891May 10, 2012Paper

Influence of environmental factors on the generation of nitric oxide in the roots of etiolated pea seedlings

Prikladnaia biokhimiia i mikrobiologiia
A K Glian'koA V Stepanov

Abstract

The article studies the nitric oxide (NO) levels in the roots of etiolated seedlings of garden peas (Pisum sativum L.) using the DAF-2DA fluorescent probe and fluorescent microscopy. Cross sections of roots of 100-150 microm (the site of a root which is 10-15 mm from the apex) are analyzed. It is shown that the level of NO in the roots after 24 h increased by more than a factor of 2 in the versions with NaNO2 and sodium nitroprusside. At feeding the seedlings with KNO3, a peak in the accumulation of NO in the roots (twofold increase) was observed after 30 min. Fertilizing seedlings with L-arginine (2 mM) increased the intensity of the fluorescence of the root sections by more than a factor of 2. The inoculation of seedlings of rhizobia (Rhizobium leguminosarum by. viceae) contributed to the reduction of NO on the background of the control (H20) and sodium nitroprusside and nitrogen compounds. Scavengers of NO (2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide (PTIO), hemoglobin) and inhibitors of nitrate reductase and animal NO synthase (sodium tungstate and aminoguanidine hydrochloride) reduced the level of NO in the roots. The results are discussed in relation to the role of NO in plants under the influence of biotic and...Continue Reading

References

Sep 21, 2000·The Plant Journal : for Cell and Molecular Biology·I FoissnerJ Durner
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M DelledonneC Lamb
Dec 13, 2001·Journal of Experimental Botany·Peter RockelWerner M Kaiser
May 9, 2002·Journal of Experimental Botany·Steven J NeillJohn T Hancock
Aug 13, 2003·Plant Physiology·Sidney L Shaw, Sharon R Long
Jan 27, 2004·The Plant Cell·Paul C BethkeRussell L Jones
Dec 27, 2005·Journal of Experimental Botany·Luis A J MurElena Prats
Dec 27, 2005·Journal of Experimental Botany·Michele PerazzolliMassimo Delledonne
Jan 7, 2006·Journal of Experimental Botany·S GrünJ Durner
Dec 1, 2006·Plant Biotechnology Journal·Madasamy ParaniStephen L Goldman
Jan 24, 2007·FEBS Letters·Raquel ValderramaJuan B Barroso
Nov 3, 2007·Journal of Experimental Botany·Steven NeillIan Wilson
Nov 23, 2007·Annual Review of Plant Biology·Angélique Besson-BardDavid Wendehenne
Mar 12, 2008·Journal of Experimental Botany·Steven NeillIan Wilson
Nov 1, 2007·Plant Signaling & Behavior·Taline Elmayan, Françoise Simon-Plas
Sep 2, 2009·Plant Signaling & Behavior·Maki NagataToshiki Uchiumi
Mar 5, 2010·Prikladnaia biokhimiia i mikrobiologiia·A K Glian'ko, G G Vasil'eva
Sep 22, 2010·Plant Physiology·Jin XuXiaojing Liu
Sep 29, 2010·Prikladnaia biokhimiia i mikrobiologiia·A K Glian'koA L Alekseenko

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