Influence of ethylene oxide sterilization on the activity of native reindeer bone morphogenetic protein

International Orthopaedics
Tarmo PekkarinenPekka Jalovaara

Abstract

We studied the effects of ethylene oxide sterilization (Steri-Vac 4XL, temperature 29 degrees C, exposure time 4 h 10 min, ethylene oxide concentration 860 mg/l) on the osteoinductivity of partially purified native reindeer bone morphogenetic protein (BMP) in a hind leg muscle pouch model of male NMRI mice. BMP was administered in implants containing 3 mg in a collagen carrier. Implants without sterilization and without BMP served as controls. New bone formation was evaluated based on the calcium yield, radiographic and histological examination 3 weeks after implantation. The implants without BMP were not able to induce new bone visible in radiographs. In the sterilized BMP group, the mean area of new bone was 35% ( p=0.004) and density 32% ( p=0.000) smaller than in the nonsterilized group. Calcium yield was 20% lower in the sterilized group than in the nonsterilized group, but this difference was not significant ( p=0.22). It was many times lower in the group without BMP than in the above-mentioned groups ( p=0,001). We conclude that ethylene oxide gas sterilization reduces the bone-forming activity of native reindeer BMP by one third.

References

Nov 12, 1965·Science·M R Urist
Dec 1, 1982·The Journal of Hospital Infection·J R BabbG A Ayliffe
Feb 1, 1981·Collagen and Related Research·A H Reddi
Sep 1, 1994·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·S IjiriK Notoya
May 29, 1998·Acta Orthopaedica Scandinavica·P Aspenberg, S B Lindqvist
May 26, 2001·International Orthopaedics·T TuominenP Jalovaara

❮ Previous
Next ❯

Citations

Feb 18, 2010·Journal of Materials Science. Materials in Medicine·Hanna TölliPekka Jalovaara
Jan 30, 2007·Acta Orthopaedica·Tarmo PekkarinenPekka Jalovaara
Jan 30, 2016·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Anjana JManitha B Nair
Feb 13, 2009·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Cornelia WiegandUta-Christina Hipler
Jun 2, 2011·Journal of Biomedical Materials Research. Part a·ZhenMing HuYan Su
Aug 29, 2007·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Bo HanMarcel Nimni
Nov 3, 2009·The Journal of Craniofacial Surgery·Ersoy KonaşHalil Ibrahim Canter
May 4, 2005·Scandinavian Journal of Surgery : SJS : Official Organ for the Finnish Surgical Society and the Scandinavian Surgical Society·T PekkarinenP Jalovaara
Mar 28, 2017·Pharmaceutical Research·Inas El BialyM Reza Nejadnik

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.