Influence of exogenous ascorbic acid and glutathione priming on mitochondrial structural and functional systems to alleviate aging damage in oat seeds

BMC Plant Biology
Fangshan XiaMingya Wang

Abstract

Loss of vigor caused by seed aging adversely affects agricultural production under natural conditions. However, priming is an economical and effective method for improving the vigor of aged seeds. The objective of this study was to test the effectiveness of exogenous ascorbic acid (ASC) and glutathione (GSH) priming in the repairing of aged oat (Avena sativa) seeds, and to test the hypothesis that structural and functional systems in mitochondria were involved in this process. Oat seeds were artificially aged for 20 days at 45 °C, and were primed with solutions (1 mmol L- 1) of ASC, GSH, or ASC + GSH at 20 °C for 0.5 h before or after their aging. Seed germination, antioxidant enzymes in the ASC-GSH cycle, cytochrome c oxidase (COX) and mitochondrial malate dehydrogenase (MDH) activities, and the mitochondrial ultrastructures of the embryonic root cells were markedly improved in aged oat seeds through post-priming with ASC, GSH, or ASC + GSH, while their malondialdehyde and H2O2 contents decreased significantly (P < 0.05). Our results suggested that priming with ASC, GSH, or ASC + GSH after aging could effectively alleviate aging damage in oat seeds, and that the role of ASC was more effective than GSH, but positive effects of ...Continue Reading

References

Jun 1, 1984·Analytical Biochemistry·B D PattersonI B Ferguson
Aug 15, 2000·Plant Science : an International Journal of Experimental Plant Biology· Madhava Rao KV, T V Sresty
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·I Iturbe-OrmaetxeM Becana
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Graham Noctor, Christine H. Foyer
Dec 16, 2004·Molecular Plant-microbe Interactions : MPMI·Maria C RubioManuel Becana
Sep 1, 1991·Plant Physiology·N R Madamanchi, R G Alscher
Aug 8, 2006·Plant Physiology and Biochemistry : PPB·Franca TommasiLaura De Gara
Jun 13, 2009·Plant & Cell Physiology·Guangkun YinXinming Jing
Sep 28, 2010·Plant Physiology and Biochemistry : PPB·Sarvajeet Singh Gill, Narendra Tuteja
Jan 6, 2011·Plant Physiology·Christine H Foyer, Graham Noctor
Sep 15, 2012·Planta·Chris CarrieJames Whelan
Sep 6, 2013·Proceedings of the National Academy of Sciences of the United States of America·Bertram DaumWerner Kühlbrandt
Dec 3, 2014·The Plant Journal : for Cell and Molecular Biology·Yu WangXiaofeng Wang
Mar 11, 2017·Plant Physiology and Biochemistry : PPB·Ying LiXiaofeng Wang
Dec 6, 2018·International Journal of Molecular Sciences·Nicolas L Taylor

❮ Previous
Next ❯

Methods Mentioned

BETA
environmental stresses
MDA
MDH
transmission electron microscopy

Software Mentioned

SPSS

Related Concepts

Related Feeds

Cell Aging (Keystone)

This feed focuses on cellular aging with emphasis on the mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.

Cell Aging

This feed focuses on cellular aging with emphasis on mitochondria, autophagy, and metabolic processes associated with aging and longevity. Here is the latest research on cell aging.