Influence of extraction solvents on antioxidant activity and the content of bioactive compounds in non-pungent peppers.

Plant Foods for Human Nutrition
Haejin BaeBhimanagouda S Patil

Abstract

Bioactive compounds in foods have been shown to maintain human health. However, the relative amounts of bioactive compounds and the variation in the amounts are still poorly understood. In this study, the efficacy of different extraction solvents (hexane, ethyl acetate, acetone, methanol, and a methanol:water mixture), as well as the levels of certain bioactive compounds in non-pungent pepper cultivars (TMH, TMJ, PA137, and B58) were investigated using high-performance liquid chromatography (HPLC). Antioxidant activities were determined using 2,2,-diphenyl-1-picrylhydrazyl (DPPH), reducing power, and deoxyribose degradation. Hexane extracts had the highest level of carotenoids (47.2-628.8 μg/g), and methanol extracts contained maximum flavonoids (24.9-152.2 μg/g) in four different cultivars. Higher DPPH scavenging activity was found in the hexane extracts from TMH, TMJ, PA137, and B58 (IC₅₀ value: 0.67, 0.74, 0.55, and 0.48 μg/ml, respectively), whereas the reducing power was high in ethyl acetate and acetone extracts. Inhibition of deoxyribose degradation was highest in methanolic extracts from TMH, TMJ, PA137, and B58 (51.2, 49.5, 52.6, and 47.4 %, respectively). These data demonstrate that solvent chemical properties such as...Continue Reading

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Citations

Feb 7, 2013·Plant Foods for Human Nutrition·Yong Seo ParkShela Gorinstein
Aug 6, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Marta O SoaresAna F Vinha
Feb 2, 2015·Plant Foods for Human Nutrition·E PeñasC Martínez-Villaluenga
Apr 1, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Katarzyna Gaweł-BębenKamila Rybczyńska
Oct 22, 2013·BMC Public Health·Violeta ChaconJoaquin Barnoya
Jul 6, 2019·Journal of Veterinary Research·Maciej KlockiewiczEwa Długosz
Aug 21, 2019·The International Journal of Behavioral Nutrition and Physical Activity·Emily Truman, Charlene Elliott
Jan 18, 2020·International Journal of Molecular Sciences·Eleonora BassinoLuca Munaron
Jan 2, 2021·International Journal of Molecular Sciences·Patrícia Ribeiro PereiraVania Margaret Flosi Paschoalin
Sep 8, 2019·Chemistry and Physics of Lipids·Sara BattistaLuisa Giansanti

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