Influence of fluorination on protein-engineered coiled-coil fibers

Biomacromolecules
Haresh T MoreJin K Montclare

Abstract

We describe the design and characterization of fluorinated coiled-coil proteins able to assemble into robust nano- and microfibers. Fluorination is achieved biosynthetically by residue-specific incorporation of 5,5,5-trifluoroleucine (TFL). The fluorinated proteins C+TFL and Q+TFL are highly α-helical as confirmed via circular dichroism (CD) and more resistant to thermal denaturation compared to their nonfluorinated counterparts, C and Q. The fluorinated proteins demonstrate enhanced fiber assembly at pH 8.0 with higher order structure in contrast to nonfluorinated proteins, which are unable to form fibers under the same conditions. Ionic strength dependent fiber assembly is observed for fluorinated as well as wild-type proteins in which the fluorinated proteins exhibited more stable, thicker fibers. The fluorinated and nonfluorinated proteins reveal metal ion-dependent small molecule recognition and supramolecular assemblies. In the presence of Zn (II), enhanced thermal stability and fiber assembly is observed for the fluorinated proteins and their nonfluorinated counterparts. Whereas Ni (II) promotes aggregation with no fiber assembly, the stabilization of α-helix by Zn (II) results in enhanced binding to curcumin by the fluo...Continue Reading

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Citations

Oct 6, 2016·Expert Opinion on Drug Delivery·Dunyin GuKatharina Ladewig
Apr 3, 2019·Advanced Healthcare Materials·Yao WangJin Kim Montclare
Jul 7, 2020·Chembiochem : a European Journal of Chemical Biology·Margeaux A Miller, Ellen M Sletten
Jan 29, 2021·Journal of Peptide Science : an Official Publication of the European Peptide Society·Michael D Jorgensen, Jean Chmielewski
Sep 9, 2019·ACS Biomaterials Science & Engineering·Priya KatyalJin K Montclare
Jul 30, 2019·Biomacromolecules·Lindsay K HillJin Kim Montclare
Mar 31, 2018·Biomacromolecules·Liming YinJin K Montclare
Sep 18, 2018·Journal of the American Chemical Society·Monessha NambiarJean Chmielewski
Jul 8, 2017·Biomacromolecules·Che Fu LiuJin Kim Montclare
Oct 29, 2021·Chemical Reviews·Nairiti J SinhaJeffery G Saven

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