PMID: 9185325May 1, 1997Paper

Influence of follicular maturation on the susceptibility of chicken ovarian granulosa cells to free oxygen radicals

Comparative Biochemistry and Physiology. Part C, Pharmacology, Toxicology & Endocrinology
G Nemecz, E K Asem

Abstract

The influence of ovarian follicular maturation on lipid peroxidation-induced changes in the fluidity of plasma membrane of granulosa cells was investigated using the fluorogenic polyunsaturated fatty acid cis-parinaric acid (cPNA). An increase in lipid peroxidation results in the decrease of fluorescence intensity of cPNA. A decrease in membrane fluidity results in the decrease of fluorescence polarization of cPNA. Granulosa cells isolated from the largest (F1; mature), and third largest (F3; developing) preovulatory follicles as well as from a pool of immature small yellow follicles (SYF) of the domestic hen ovary were pre-labelled with cPNA. The cPNA-labelled cells were exposed to a free oxygen radical generating agent 2,2'azobis-(2-amidinopropane)-hydrochloride (AAPH). AAPH enhanced the rate of decrease in fluorescence intensity of cPNA-labelled cells. This effect of AAPH was greater in the more differentiated granulosa cells obtained from mature follicles than in less differentiated cells isolated from immature follicles. Similarly, the degree of change in fluorescence polarization was greater in more differentiated F1 granulosa cells than in the less differentiated ones. The analysis of fatty acid composition of phospholip...Continue Reading

References

Aug 15, 1992·Archives of Biochemistry and Biophysics·J A LaranjinhaV M Madeira
Jan 1, 1991·Free Radical Biology & Medicine·J J van den BergJ A Op den Kamp
Dec 1, 1991·Proceedings of the Society for Experimental Biology and Medicine·J C Riley, H R Behrman
Dec 1, 1986·Biology of Reproduction·F E Robinson, R J Etches
Sep 25, 1987·Biochimica Et Biophysica Acta·F A KuypersJ A Op den Kamp
Feb 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·M Sinensky
Apr 1, 1981·Archives of Biochemistry and Biophysics·G NemeczL I Horváth

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