PMID: 9166286May 1, 1997Paper

Influence of glucose on murine metanephric development and proteoglycans: morphologic and biochemical studies

Laboratory Investigation; a Journal of Technical Methods and Pathology
Y S KanwarE I Wallner

Abstract

The offspring of severe juvenile diabetics suffer from a multitude of congenital anomalies, including genito-urinary defects. Whether these defects are related to hyperglycemic states remains to be determined. In this study, the effect of glucose on metanephric development and extracellular matrix proteoglycans (PG), the regulators of morphogenesis, was investigated. Metanephric explants, harvested at Day 13 of gestation, were exposed to 30 mM of D-glucose for 1 to 7 days in an organ culture system. Light microscopy revealed a significant reduction in the size of explants and the nephron population in metanephroi exposed to glucose. A marked dysmorphogenesis of the ureteric bud branches was also observed. They were swollen and had blunted tips. The latter are the site of nascent nephron formation. Electron microscopy revealed malformation of the S-shaped body nephrons, which had poorly formed clefts and lacked cells in their distal convolutions. The precapillary stage glomeruli showed effacement of the foot processes, attenuation of the glomerular basement membrane, decreased surface microvilli, and an increased number of intercellular junctions. Immunofluorescence microscopy indicated a decreased reactivity of antibody directe...Continue Reading

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