Influence of glycerol on the structure and thermal stability of lysozyme: a dynamic light scattering and circular dichroism study

The Journal of Physical Chemistry. B
Alessandro EspositoGiuseppe Onori

Abstract

Photon correlation spectroscopy and circular dichroism have been used to study the role of hydration in the structure and thermostability of the model protein lysozyme in water-glycerol mixtures. Two cases have been considered: water-rich and glycerol-rich regimes of concentrations. We follow the thermal denaturation both by optical spectral changes and hydrodynamic radius variations. This methodology allows us to emphasize the relevant role played by hydrophobic interactions during the process in aqueous solutions and, in glycerol, to distinguish the non-cooperative melting of secondary structure, supporting the view of a protein transition to a molten globule-like state.

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Citations

Jun 1, 2013·Physical Chemistry Chemical Physics : PCCP·Rocio EsquembreM Luisa Ferrer
Jan 14, 2012·The Journal of Chemical Physics·Christoph GögeleinStefan U Egelhaaf
Dec 18, 2013·The Journal of Chemical Physics·Paola SassiAssunta Morresi
Aug 21, 2014·Journal of Pharmaceutical Sciences·Louise Stenstrup HolmMarco van de Weert
Dec 3, 2014·Journal of Molecular Biology·Dorota KubackaJoanna Zuberek
May 25, 2011·Biophysical Chemistry·Paola SassiGiuseppe Onori
Jul 3, 2010·Biochemical and Biophysical Research Communications·Honglin LiuShiqiang Wei
Apr 25, 2012·FEMS Microbiology Letters·Jaeeun ParkSangryeol Ryu
Nov 18, 2011·Physical Chemistry Chemical Physics : PCCP·Katherine D WeaverGloria D Elliott
Jun 5, 2020·International Journal of Biological Macromolecules·Timur MagsumovIgor Sedov
Feb 14, 2013·The Journal of Physical Chemistry. B·Alessandra GiugliarelliLaurence Nafie

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