[Influence of glyoxylic acid on properties of isolated mitochondria].

Biochimie
M Lucas, A M Pons

Abstract

Glyoxalate is an effector of oxidative phosphorylation in isolated mitochondria : it slows down State 3 but does not affect State 4 respiration. This report presents the findings of our study on the mechanism of action of glyoxalate ; these findings are listed below. The inhibition of Stage 3 respiration by glyoxalate does not set in immediately, can be reversed in part by the addition of an uncoupling agent or a dithiol, is non-competitive against succinate and can be demonstrated with substrates requiring the involvement of other membrane transport systems. Glyoxalate prevents the increased oxygen uptake stimulated by 2,4-DNP or Sr++. Glyoxalate also inhibits phosphate transport and this inhibition can account for most of the effect observed. The inhibition of State 3 respiration is paralleled by a decrease in the mitochondrial accumulation of succinate : this decrease could arise from a direct effect of glyoxalate on dicarboxylic acid transport or could be the result of an inhibiton of the phosphate transport system, which is connected with the former. The decrease in the respiratory rate of uncoupled mitochondria placed in a phosphate free medium demonstrates that the effector acts directly at the substrate transport or/and...Continue Reading

References

Apr 1, 1969·European Journal of Biochemistry·F Palmieri, E Quagliariello
Jan 1, 1969·Annual Review of Biochemistry·H A Lardy, S M Ferguson
Mar 1, 1968·European Journal of Biochemistry·E Quagliariello, F Palmieri
Jul 21, 1967·Biochemical and Biophysical Research Communications·B H Robinson, J B Chappell
Feb 1, 1970·The Biochemical Journal·A J MeijerE Quagliariello
Feb 16, 1970·FEBS Letters·M Klingenberg
Jan 1, 1961·Advances in Clinical Chemistry·J NORDMANN, R NORDMANN

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