Influence of growth substrate on production of cellulase enzymes by Trichoderma harzianum E58

Biotechnology and Bioengineering
M Mes-HartreeJ N Saddler

Abstract

Cellulase production by Trichoderma harzianum E58 grown on lactose and various cellulosic substrates such as Solka Floe, Avicel, and steamed aspenwood was investigated. The culture filtrates of T. harzianum E58 obtained after growth on these substrates were assayed for their cellulase activities and overall hydrolytic activities. The severity of the steaming conditions used for the aspenwood had a pronounced effect on the cellulolytic activity of the produced culture filtrates. Those substrates that were more readily hydrolyzed by the cellulase complex were the poorest substrates for inducing an active cellulase complex. Substrates such as acid-impregnated aspenwood and lactose induced a less hydrolytic efficient cellulase complex than more recalcitrant substrates such as microcrystalline cellulose.

References

Dec 1, 1971·Journal of General Microbiology·M A Hulme, D W Stranks
Jan 1, 1960·Scandinavian Journal of Clinical and Laboratory Investigation·E RAABO, T C TERKILDSEN
Feb 1, 1962·Journal of Bacteriology·M MANDELSE T REESE
Jun 1, 1982·Biotechnology and Bioengineering·J N SaddlerN Levitin
Apr 1, 1986·Biotechnology and Bioengineering·R San MartinA F Sciamanna

Related Concepts

Acids
Cellulase
Cellulose
Lactose
UL98 protein, cytomegalovirus
Microcrystalline Cellulose
Avicel
Cellulases
Trichoderma harzianum

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