Influence of guanine nucleotides on complex formation between Ras and CDC25 proteins.

Molecular and Cellular Biology
C C LaiS Powers

Abstract

The Saccharomyces cerevisiae CDC25 gene and closely homologous genes in other eukaryotes encode guanine nucleotide exchange factors for Ras proteins. We have determined the minimal region of the budding yeast CDC25 gene capable of activity in vivo. The region required for full biological activity is approximately 450 residues and contains two segments homologous to other proteins: one found in both Ras-specific exchange factors and the more distant Bud5 and Lte1 proteins, and a smaller segment of 48 amino acids found only in the Ras-specific exchange factors. When expressed in Escherichia coli as a fusion protein, this region of CDC25 was found to be a potent catalyst of GDP-GTP exchange on yeast Ras2 as well as human p21H-ras but inactive in promoting exchange on the Ras-related proteins Ypt1 and Rsr1. The CDC25 fusion protein catalyzed replacement of GDP-bound to Ras2 with GTP (activation) more efficiently than that of the reverse reaction of replacement of GTP for GDP (deactivation), consistent with prior genetic analysis of CDC25 which indicated a positive role in the activation of Ras. To more directly study the physical interaction of CDC25 and Ras proteins, we developed a protein-protein binding assay. We determined that...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D BowtellP Senior
Jan 31, 1992·Science·L BonfiniU Banerjee
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·F R Bischoff, H Ponstingl
Mar 22, 1990·Nature·D A HughesM Yamamoto
Jan 1, 1986·Annual Review of Neuroscience·L Stryer
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·A Bender, J R Pringle

❮ Previous
Next ❯

Citations

Sep 25, 2014·International Journal of Molecular Sciences·Xueqing BaIstvan Boldogh
Oct 19, 2000·The Journal of Cell Biology·W WangS Ferro-Novick
Dec 23, 2015·Nature Genetics·Jessica OkosunJude Fitzgibbon
Feb 10, 1997·FEBS Letters·S Hermann-Le Denmat, M Jacquet
Dec 31, 2009·Cellular Microbiology·Ana ArbeloaGad Frankel
Nov 30, 2010·Biochimica Et Biophysica Acta·Alberto Fernández-Medarde, Eugenio Santos
Jul 18, 2009·BMC Systems Biology·Thomas WilliamsonLubomira Stateva
Apr 10, 1999·Structure·J J DumasD G Lambright
Jul 11, 2006·Developmental Cell·Matthias P Machner, Ralph R Isberg
Oct 22, 2008·Biochimica Et Biophysica Acta·Renata TisiEnzo Martegani
Aug 12, 1998·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J Y Pan, M Wessling-Resnick
Apr 28, 2005·Oncogene·Aurélien G DupuyJean de Gunzburg
Feb 15, 1995·European Journal of Biochemistry·M SegalA Levitzki
Jul 19, 1996·The Journal of Biological Chemistry·J B CréchetA Parmeggiani
May 10, 1996·The Journal of Biological Chemistry·L A QuilliamC J Der
Dec 5, 2000·Molecular Biology of the Cell·S JonesN Segev
Aug 5, 1998·Nature·P A Boriack-SjodinJ Kuriyan
Sep 1, 1999·European Journal of Biochemistry·P MeyerS Baudet-Nessler
Aug 3, 2004·Genesis : the Journal of Genetics and Development·Serena J SilverIlaria Rebay
May 10, 1994·Molecular & General Genetics : MGG·C Costigan, M Snyder
May 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·L A QuilliamC J Der
Dec 16, 1993·Nature·M S Boguski, F McCormick
Jan 31, 2019·Cellular Microbiology·Adela Martin-VicenteJarrod R Fortwendel
Jul 3, 2019·The Plant Journal : for Cell and Molecular Biology·Monika KaldeFarhah F Assaad
Nov 21, 2014·Molecular Systems Biology·Ilya Soifer, Naama Barkai
Jun 5, 2003·The Journal of Cell Biology·Satoshi YoshidaAkio Toh-e

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.