Influence of Helicobacter pylori culture supernatant on the ecological balance of a dual-species oral biofilm

Journal of Applied Oral Science : Revista FOB
Wenling ZhangYuqing Li

Abstract

Dental caries is a chronic progressive disease occurring in the tooth hard tissue due to multiple factors, in which bacteria are the initial cause. Both Streptococcus mutans and Streptococcus sanguinis are main members of oral biofilm. Helicobacter pylori may also be detected in dental plaque, playing an important role in the development of dental caries. The aim of this study was to investigate the effect of H. pylori culture supernatant on S. mutans and S. sanguinis dual-species biofilm and to evaluate its potential ability on affecting dental health. The effect of H. pylori supernatant on single-species and dual-species biofilm was measured by colony forming units counting and fluorescence in situ hybridization (FISH) assay, respectively. The effect of H. pylori supernatant on S. mutans and S. sanguinis extracellular polysaccharides (EPS) production was measured by both confocal laser scanning microscopy observation and anthrone-sulfuric acid method. The effect of H. pylori supernatant on S. mutans gene expression was measured by quantitative real-time PCR (qRT-PCR) assays. H. pylori supernatant could inhibit both S. mutans and S. sanguinis biofilm formation and EPS production. S. sanguinis inhibition rate was significantly ...Continue Reading

References

Nov 1, 1991·Scandinavian Journal of Gastroenterology·H G DesaiS R Prabhu
Jan 5, 2002·Scandinavian Journal of Infectious Diseases·K L KolhoH Rautelin
Jun 12, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·S A Dowsett, M J Kowolik
May 22, 2004·Caries Research·P D Marsh
Sep 21, 2005·Archives of Otolaryngology--head & Neck Surgery·Mohamed Nasser Elsheikh, Magdy ElSayed Mahfouz
Apr 26, 2006·Journal of Clinical Periodontology·E C E GebaraL A P A Lima
Oct 25, 2006·British Journal of Biomedical Science·S I SmithA O Coker
May 16, 2009·Archives of Oral Biology·Denise G SilvaEduardo M B Tinoco
May 25, 2010·Nature Reviews. Cancer·D Brent Polk, Richard M Peek
Oct 12, 2010·Clinical Microbiology Reviews·Lydia E WroblewskiKeith T Wilson
Feb 8, 2011·Journal of Oral Pathology & Medicine : Official Publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology·Qing-Hua Zou, Ren-Qing Li
Jul 12, 2011·Journal of Dental Research·E G Smith, G A Spatafora
May 9, 2013·Nature Reviews. Microbiology·Nina R SalamaAnne Müller
Aug 30, 2013·Asia-Pacific Journal of Public Health·Peng LiuDong Chen
Sep 21, 2013·Journal of Dental Research·H KooM I Klein
Mar 29, 2014·Journal of Applied Oral Science : Revista FOB·Juliana Paola Correa da SilvaIvana Barbosa Suffredini
Jul 16, 2014·Journal of Applied Oral Science : Revista FOB·Julia Caroline SchmidtClemens Walter
Dec 4, 2014·Journal of Applied Oral Science : Revista FOB·Michele BarnabéIvana Barbosa Suffredini
Mar 13, 2015·Frontiers in Cellular and Infection Microbiology·Marlise I KleinHyun Koo
Sep 9, 2015·Future Microbiology·Ming-Yi WangXiao-Zhong Gao
Oct 21, 2015·Antimicrobial Agents and Chemotherapy·Zhi RenYuqing Li
Aug 2, 2016·Molecular Oral Microbiology·J KrethJ Merritt

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Citations

May 28, 2019·World Journal of Microbiology & Biotechnology·Jun LuLiming Zhao
May 16, 2018·Materials·Ilaria ArmentanoLuigi Torre
Apr 10, 2020·The Journal of Clinical Pediatric Dentistry·Hisham Yehia El BatawiSaaid Al Shehadat
Feb 6, 2019·European Journal of Microbiology & Immunology·Hagen FrickmannAndreas Podbielski

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Methods Mentioned

BETA
confocal microscopy
PCR

Software Mentioned

IMAGE PRO PLUS
SPSS

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