Influence of human immunodeficiency virus nucleocapsid protein on synthesis and strand transfer by the reverse transcriptase in vitro.

The Journal of Biological Chemistry
L Rodríguez-RodríguezP J Fay

Abstract

Human immunodeficiency virus (HIV) nucleocapsid protein (NC) influences HIV reverse transcriptase (RT) catalyzed strand transfer synthesis from internal regions of natural sequence RNA. In the strand transfer assay reaction in vitro, primer synthesis initiated on a donor template can transfer and be completed on an acceptor template. NC was added at concentrations up to twice that needed for 100% template coating. As the concentration of NC was increased, primer extension was stimulated until NC coated approximately 50% of the template. Stimulation was caused in part by an increase in the number of primers that sustained synthesis. Subsequent increments of NC decreased synthesis. The presence of NC also increased the efficiency of the strand transfer reaction, allowing a greater proportion of extended primers to transfer from donor to acceptor templates. Processivity of the RT on the donor template was measured using both challenged and enzyme dilution assays. NC did not alter the proportion of synthesis products that reached the end of the template, indicating little effect on processivity. This result suggests that the increase in full-length product synthesis, observed in reactions where the RT repeatedly bound the primer-te...Continue Reading

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·H De RocquignyJ L Darlix
Nov 30, 1990·Science·W S Hu, H M Temin
Feb 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·W S Hu, H M Temin
Nov 25, 1988·Science·B D PrestonL A Loeb
Nov 25, 1988·Science·J D RobertsT A Kunkel
Jan 1, 1987·AIDS Research and Human Retroviruses·F D VeroneseM G Sarngadharan
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·W G RiceM R Fesen
Oct 1, 1993·Science·Z TsuchihashiD Herschlag
Feb 1, 1993·Protein Science : a Publication of the Protein Society·F Dib-HajjD P Giedroc

❮ Previous
Next ❯

Citations

Mar 21, 2000·Medicinal Research Reviews·H JonckheereE De Clercq
Dec 1, 1996·Reviews in Medical Virology·W G Rice, J A Turpin
Jun 7, 1996·Biochimica Et Biophysica Acta·M P Pop
Mar 30, 1999·Biochimica Et Biophysica Acta·B I KlasensB Berkhout
Dec 8, 2009·Nature Structural & Molecular Biology·Dorota Piekna-PrzybylskaRobert A Bambara
Nov 26, 2010·Nature Structural & Molecular Biology·Shixin LiuXiaowei Zhuang
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·P J TumminoD Hupe
Nov 25, 1998·Nucleic Acids Research·R E JeeningaB Berkhout
Sep 15, 2001·Nucleic Acids Research·A MoumenM Negroni
Aug 9, 2003·Nucleic Acids Research·Nick LeeKarin Musier-Forsyth
Sep 23, 2003·Nucleic Acids Research·Sébastien LyonnaisGilles Mirambeau
Feb 14, 2006·Journal of Virology·Christian Lanciault, James J Champoux
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·M Negroni, H Buc
Apr 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·S DruillennecM C Fournié-Zaluski
Jun 26, 2010·Physics of Life Reviews·Kathy R ChaurasiyaMark C Williams
Nov 15, 2008·Science·Stefan G Sarafianos, Eddy Arnold
Feb 19, 2008·Virus Research·Vandana Purohit BasuRobert A Bambara
Oct 31, 2006·Journal of Molecular Biology·Reshma M Anthony, Jeffrey J Destefano
Oct 12, 2005·Journal of Molecular Biology·Mark Nils HansonRobert A Bambara
Oct 27, 2009·Journal of Molecular Biology·Sean T RigbyRobert A Bambara

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.