Influence of Hydrophobic Face Amino Acids on the Hydrogelation of β -Hairpin Peptide Amphiphiles

Macromolecules
Christopher M MicklitschJoel P Schneider

Abstract

Hydrophobic residues provide much of the thermodynamic driving force for the folding, self-assembly, and consequent hydrogelation of amphiphilic β-hairpin peptides. We investigate how the identity of hydrophobic side chains displayed from the hydrophobic face of these amphiphilic peptides influences their behavior to expound on the design criteria important to gel formation. Six peptides were designed that globally incorporate valine, aminobutyric acid, norvaline, norleucine, phenylalanine, or isoleucine on the hydrophobic face of the hairpin to study how systematic changes in hydrophobic content, β-sheet propensity, and aromaticity affect gelation. Circular dichroism (CD) spectroscopy indicates that hydrophobic content, rather than β-sheet propensity, dictates the temperature- and pH-dependent folding and assembly behavior of these peptides. Transmission electron microscopy (TEM) and small-angle neutron scattering (SANS) show that the local morphology of the fibrils formed via self-assembly is little affected by amino acid type. However, residue type does influence the propensity of peptide fibrils to undergo higher order assembly events. Oscillatory rheology shows that the mechanical rigidity of the peptide gels is highly inf...Continue Reading

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Citations

Jan 21, 2021·Frontiers in Bioengineering and Biotechnology·Galit Fichman, Joel P Schneider
Aug 5, 2016·Chemical Society Reviews·Juan WangXuehai Yan
Sep 1, 2016·Nanotechnology·Melis Sardan EkizMustafa O Guler
Jun 16, 2016·Chemical Society Reviews·Luis M De Leon RodriguezMargaret A Brimble
Jul 4, 2018·Angewandte Chemie·Junfeng ShiJoel P Schneider
Apr 21, 2018·Chemical Communications : Chem Comm·Mingtao TaoWenying Zhong
Mar 5, 2017·Advanced Drug Delivery Reviews·Peter WorthingtonDarrin Pochan
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Galit Fichman, Joel P Schneider
Apr 27, 2021·Accounts of Chemical Research·Jevgenij A RaskatovBradley L Nilsson
Mar 6, 2018·Biomacromolecules·Yuji YamadaWilliam G Stetler-Stevenson
Jun 21, 2017·Biomacromolecules·Laura L E MearsDave J Adams
Oct 4, 2018·Chemical Reviews·Corey J WilsonJennifer M Heemstra
Oct 2, 2021·Langmuir : the ACS Journal of Surfaces and Colloids·Yanqing GeJiqian Wang

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