Influence of hypertension on cardiac contractile response of human erythrocyte-derived depressing factor in ventricular myocytes

Journal of Hypertension
Jun RenKadon K Hintz

Abstract

Erythrocyte-derived depressing factor (EDDF), a novel hypotensive factor purified from human erythrocytes, elicits endothelium-dependent vasorelaxation by reducing intracellular Ca2+ in vascular smooth muscle cells. However, its cardiac response is unknown. This study was designed to examine the cardiac contractile response of EDDF under both normotensive and hypertensive conditions. Ventricular myocytes were isolated from adult male spontaneously hypertensive rats (SHR) and age-matched Wistar-Kyoto (WKY) normotensive rats. Mechanical properties were evaluated using an IonOptix MyoCam system and intracellular Ca2+ was measured with fura-2 fluorescence. Myocytes were electrically stimulated to contract at 0.5 Hz. The contractile properties analyzed included peak shortening (PS), time-to-PS (TPS), time-to-90% re-lengthening (TR(90)), maximal velocity of shortening/re-lengthening (+/- dl/dt), fura-fluorescence intensity change (DeltaFFI), and fura-fluorescence decay rate (tau). SHR rats displayed significantly elevated blood pressure. EDDF (10-9-10-4 g/ml) did not affect PS, TPS, TR(90), DeltaFFI and tau but depressed +/- dl/dt at higher doses in WKY myocytes. However, EDDF depressed PS, +/- dl/dt and DeltaFFI, shortened TPS witho...Continue Reading

References

Jan 1, 1995·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·C M Fernández-RodriguezJ Penas
Jul 1, 1995·International Journal of Cardiology·P MohanD L Brutsaert
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Mar 23, 2001·Pathology, Research and Practice·Y NakanumaK Katayanagi
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Citations

Jun 5, 2003·Journal of Hypertension·Giulio SelvetellaGiuseppe Lembo
Apr 19, 2007·Clinical and Experimental Pharmacology & Physiology·Huan PangLei Shi

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