Influence of IL-12 on interferon-gamma production by bovine leucocyte subsets in response to bovine respiratory syncytial virus

Veterinary Immunology and Immunopathology
R A CollinsC J Howard

Abstract

The cytokine IL-12 is a key molecule in the regulation of CD4+ T cell development and specifically potentiates the development of T helper 1 responses in mouse and man. However the biological effects mediated by bovine IL-12 have not been defined in cattle. To produce the expression of the two mature proteins a polyprotein approach was used. This system is employed by positive strand viruses and encodes both products from a single open reading frame (ORF). The 2A region of foot-and-mouth disease virus (FMDV) encodes a site that appears to undergo auto-cleavage. Here the 2A was flanked by sequences encoding the p35 and p40 polypeptides of the heterodimeric cytokine to mediate their cleavage. Formation of the correct heterodimeric structure is an absolute requirement for IL-12 biologic activity. Using bovine respiratory syncytial virus (BRSV) and ovalbumin (OVA) we studied the effects of IL-12 on the responses of peripheral blood mononuclear cells (PBMC) to these antigens, in vitro. The presence of IL-12 markedly influenced the level of IFNg secreted by these cells, and although IL-12 induced IFNg production in the absence of antigenic stimulation, IFNg production was accelerated and augmented in response to IL-12 and antigen. An...Continue Reading

References

Feb 13, 1996·Annals of the New York Academy of Sciences·M YamamotoJ R McGhee

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Citations

Oct 18, 2002·Veterinary Immunology and Immunopathology·G I Solano-AguilarJ K Lunney
Aug 7, 1999·Veterinary Immunology and Immunopathology·R A CollinsD Werling
Aug 24, 1999·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·W TuoW C Brown
Jan 1, 2010·Biotechnology & Genetic Engineering Reviews·Garry LukeMartin Ryan
Apr 2, 2003·Journal of Veterinary Medicine. B, Infectious Diseases and Veterinary Public Health·A M AlluwaimiJ S Cullor
May 13, 2004·Clinical and Diagnostic Laboratory Immunology·Douglas D BannermanPascal Rainard
Nov 30, 2018·Frontiers in Microbiology·Gisselle N MedinaTeresa de Los Santos
Feb 13, 2001·Cytokine·C MurphyM Giese
Jul 21, 2001·Tuberculosis·P R Wood, S L Jones
Feb 5, 2005·Veterinary Immunology and Immunopathology·Susanne Nedergaard GrellPeter Mikael Helweg Heegaard
Jun 23, 2005·Veterinary Immunology and Immunopathology·Douglas D BannermanJayne C Hope

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