Influence of indicating enzyme reaction on apparent creatine kinase activity creatine kinase in serum, VII

Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Für Klinische Chemie Und Klinische Biochemie
G SzászW Gruber

Abstract

Comparison of the indicative systems yeast glucose-6-phosphate dehydrogenase/NADP+, leuconostoc glucose-6-phosphate dehydrogenase/NADP+ and leuconostoc glucose-6-phosphate dehydrogenase/NAD+ showed excellent correlation and no differences in apparent creatine kinase activity with the two methods using NADP+. By using NAD+ with the leuconostoc enzyme enzyme relative recovery of apparent creatine kinase activity is lower due to interference of other serum constituents. The mean value of the relative differences versus methods using NADP+ was 5.8% in our experiments.

Citations

Apr 30, 1985·Clinica Chimica Acta; International Journal of Clinical Chemistry·R J KraaijenhagenE T Backer

Related Concepts

Macro-Creatine Kinase
Glucosephosphate Dehydrogenase
Leuconostoc
NADP

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