Influence of Indomethacin on Steroid Metabolism: Endocrine Disruption and Confounding Effects in Urinary Steroid Profiling of Anti-Doping Analyses

Metabolites
Anna StollMaria Parr

Abstract

Anabolic androgenic steroids (AAS) are prohibited as doping substances in sports by the World Anti-Doping Agency. Concentrations and concentration ratios of endogenous AAS (steroid profile markers) in urine samples collected from athletes are used to detect their administration. Certain (non-prohibited) drugs have been shown to influence the steroid profile and thereby sophisticate anti-doping analysis. It was shown in vitro that the non-steroidal anti-inflammatory drug (NSAID) indomethacin inhibits selected steroid-biotransformations catalyzed by the aldo-keto reductase (AKR) 1C3, which plays a key role in the endogenous steroid metabolism. Kinetic parameters for the indomethacin-mediated inhibition of the AKR1C3 catalyzed reduction in etiocholanolone were determined in vitro using two comparing methods. As NSAIDs are very frequently used (not only) by athletes, the inhibitory impact of indomethacin intake on the steroid metabolism was evaluated, and steroid profile alterations were detected in vivo (one male and one female volunteer). Significant differences between samples collected before, during or after the intake of indomethacin for selected steroid profile markers were observed. The presented results are of relevance fo...Continue Reading

References

Feb 1, 1975·Journal of Reproduction and Fertility·S K SaksenaM C Chang
Jun 24, 2008·Journal of Mass Spectrometry : JMS·Ute MareckWilhelm Schänzer
Aug 12, 2009·British Journal of Sports Medicine·T GorskiL F Martins Kruel
Oct 9, 2009·The American Journal of Sports Medicine·Philippe TschollJiri Dvorak
Feb 4, 2010·Endocrinology and Metabolism Clinics of North America·Pierre-Edouard SottasChristophe Saudan
Nov 18, 2010·Environmental Health Perspectives·David M KristensenHenrik Leffers
Dec 25, 2012·Chemico-biological Interactions·Nataša BeraničTea Lanišnik Rižner
Jan 23, 2013·Drug Testing and Analysis·D Martínez-BritoM Granda Fraga
Sep 14, 2013·The Journal of Clinical Endocrinology and Metabolism·Séverine Mazaud-GuittotBernard Jégou
Oct 12, 2014·Chemico-biological Interactions·Trevor M Penning
Dec 18, 2014·Drug Testing and Analysis·Xavier de la TorreFrancesco Botrè
Oct 21, 2016·Drug Testing and Analysis·Jenny E MullenLena Ekström
Feb 27, 2018·Journal of Toxicologic Pathology·Chikuma Hamada
May 12, 2020·Journal of Analytical Toxicology·Anna StollMaria Parr

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Citations

Nov 18, 2021·Drug Testing and Analysis·Mario ThevisHans Geyer

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Software Mentioned

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