Influence of interleukin 1alpha on superoxide anion, platelet activating factor release and phospholipase A2 activity of naive and sensitized guinea-pig alveolar macrophages

British Journal of Pharmacology
S MugnaiL Zilletti

Abstract

1. We studied the effect exerted by hr-interleukin-1alpha (IL-1alpha) on responsiveness of alveolar macrophages (AM) from naive and sensitized guinea-pigs, through O2.- production (by ferricytochrome C reduction), platelet-activating factor (PAF) release (by platelet aggregation), prostaglandin E2 (PGE2) release (by a radioimmunoassay), and cytosolic phospholipase A2 (cPLA2) activity (by hydrolysis of radioactive substrate). 2. In naive guinea-pig AM, 0.06 nM hr-IL-1alpha pretreatment decreased by 65% O2.- release stimulated with 10 nM fMLP. In contrast, O2.- production was not affected in sensitized guinea-pig AM. 3. O2.- release elicited by fMLP stimulation in both cell groups was affected by PLA2 inhibitors (10 microM bromophenacyl bromide, BPB or 10 microM methylprednisolone, MP). In contrast, 10 microM arachidonyl trifluoromethyl ketone (AACOCF3), a cPLA2 inhibitor, was ineffective. 4. In naive AM, PAF release was elicited by hr-IL-1alpha pretreatment and by separate fMLP-stimulation, but when the stimulus was added to hr-IL-1alpha-pretreated cells inhibition of PAF release was observed. In sensitized AM, PAF release was lower than that found in naive guinea-pig AM in both hr-IL-1alpha-pretreated and fMLP-stimulated cells....Continue Reading

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Citations

Jan 7, 2004·Journal of Photochemistry and Photobiology. B, Biology·Mario CiuffiPaola Failli
Nov 8, 2001·European Journal of Pharmacology·C HornussK Racké
Feb 9, 2000·Pharmacology & Therapeutics·A T Demiryürek, R M Wadsworth
Mar 26, 2002·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Yorimasa OgataHiroshi Sugiya
Jul 1, 2011·The Journal of Biological Chemistry·Mariana Morato-MarquesCarlos H Serezani
Jun 17, 2008·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Emanuela MasiniPier Francesco Mannaioni

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