Influence of ionic strength changes on the structure of pre-adsorbed salivary films. A response of a natural multi-component layer

Colloids and Surfaces. B, Biointerfaces
Lubica MacakovaJason R Stokes

Abstract

Salivary films coating oral surfaces are critically important for oral health. This study focuses on determining the underlying nature of this adsorbed film and how it responds to departures from physiological conditions due to changes in ionic strength. Under physiological conditions, it is found that pre-adsorbed in vitro salivary film on hydrophobic surfaces is present as a highly hydrated viscoelastic layer. We follow the evolution of this film in terms of its effective thickness, hydration and viscoelastic properties, as well as adsorbed mass of proteins, using complementary surface characterisation methods: a Surface Plasmon Resonance (SPR) and a Quartz Crystal Microbalance with Dissipation Monitoring (QCM-D). Our results support a heterogeneous model for the structure of the salivary film with an inner dense anchoring layer and an outer highly extended hydrated layer. Further swelling of the film was observed upon decreasing the salt concentration down to 1mM NaCl. However, upon exposure to deionised water, a collapse of the film occurs that was associated with the loss of water contained within the adsorbed layer. We suggest that the collapse in deionised water is driven by an onset of electrostatic attraction between d...Continue Reading

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Citations

Sep 29, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Javier SotresThomas Arnebrant
Oct 30, 2012·Colloids and Surfaces. B, Biointerfaces·A AshP J Wilde
Sep 11, 2012·Journal of Colloid and Interface Science·Alejandro BarrantesThomas Arnebrant
Sep 27, 2012·Colloids and Surfaces. B, Biointerfaces·Jiangwu YaoFeng Lin
Feb 22, 2011·Journal of Colloid and Interface Science·Adam L J OlssonPrashant K Sharma
Aug 18, 2012·Journal of Molecular Recognition : JMR·Robert E Speight, Matthew A Cooper
Jan 20, 2011·European Journal of Oral Sciences·Deepak H VeeregowdaPrashant K Sharma
Apr 8, 2014·Microscopy Research and Technique·Martine MorzelJeannine Lherminier
Aug 1, 2012·Carbohydrate Polymers·Marta WestwoodVictor J Morris
Apr 7, 2015·Langmuir : the ACS Journal of Surfaces and Colloids·Xiang WangQiang Zheng
Jun 13, 2014·Colloids and Surfaces. B, Biointerfaces·Hannah L GibbinsGuy H Carpenter
Nov 8, 2014·Biofouling·Watcharapong AroonsangLiselott Lindh
Jan 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Francis CanonBernard Cabane
Nov 29, 2016·Food & Function·S A RodriguesB J James
Mar 21, 2015·PloS One·Hannah L GibbinsGuy H Carpenter
Jan 26, 2013·Chemical Society Reviews·Hui Jiang, Fu-Jian Xu
Mar 4, 2017·Journal of Molecular Recognition : JMR·Christian SpenglerKarin Jacobs
Oct 8, 2011·Journal of Dental Research·F RuppC von Ohle
May 16, 2019·Journal of Texture Studies·Michael W BoehmStefan K Baier
Jun 19, 2019·Bioinspiration & Biomimetics·Clementine PradalJason R Stokes
Jun 1, 2019·Journal of Texture Studies·Heather M ShewanJason R Stokes
Sep 14, 2019·Advances in Colloid and Interface Science·Anwesha SarkarSeunghwan Lee
Feb 3, 2021·International Journal of Molecular Sciences·Paulina KomorekBarbara Jachimska
May 29, 2020·ACS Applied Materials & Interfaces·Hongping WanPrashant K Sharma
Mar 4, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Jason R StokesHarmen H J de Jongh
Jul 13, 2019·Journal of Agricultural and Food Chemistry·Michael W BoehmStefan K Baier
Oct 28, 2020·ACS Applied Materials & Interfaces·Efren Andablo-ReyesAnwesha Sarkar
Jul 3, 2018·Journal of Agricultural and Food Chemistry·Francis CanonElisabeth Guichard

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