Influence of Isoflurane on Immediate-Early Gene Expression

Frontiers in Behavioral Neuroscience
Kristopher M BuntingAlmira Vazdarjanova

Abstract

Anterograde amnesia is a hallmark effect of volatile anesthetics. Isoflurane is known to affect both the translation and transcription of plasticity-associated genes required for normal memory formation in many brain regions. What is not known is whether isoflurane anesthesia prevents the initiation of transcription or whether it halts transcription already in progress. We tested the hypothesis that general anesthesia with isoflurane prevents learning-induced initiation of transcription of several memory-associated immediate-early genes (IEGs) correlated with amnesia; we also assessed whether it stops transcription initiated prior to anesthetic administration. Using a Tone Fear Conditioning paradigm, rats were trained to associate a tone with foot-shock. Animals received either no anesthesia, anesthesia immediately after training, or anesthesia before, during, and after training. Animals were either sacrificed after training or tested 24 h later for long-term memory. Using Cellular Compartment Analysis of Temporal Activity by Fluorescence in situ Hybridization (catFISH), we examined the percentage of neurons expressing the IEGs Arc/Arg3.1 and Zif268/Egr1/Ngfi-A/Krox-24 in the dorsal hippocampus, primary somatosensory cortex, an...Continue Reading

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Citations

Aug 25, 2018·Proceedings of the National Academy of Sciences of the United States of America·Abu Shufian Ishtiaq AhmedJiliang Zhou
Oct 22, 2019·Frontiers in Integrative Neuroscience·Dong LiClaus C Hilgetag

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Methods Mentioned

BETA
Fluorescence
catFISH
in vitro transcription

Software Mentioned

StatView

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